Team:IIT Madras/Notebook

From 2011.igem.org

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<h2>Week 1</h2>
<h2>Week 1</h2>
<li>Metabolic simulation and modelling of increase in proton flux on addition of Proteorhodopsin
<li>Metabolic simulation and modelling of increase in proton flux on addition of Proteorhodopsin
-
<li>Literature survey on expression of Proteorhodopsin in E.coli</br></br>
+
<li>Literature survey on expression of Proteorhodopsin in E.coli<br>
<h2>Week 2</h2>
<h2>Week 2</h2>
<li>Minimal media characterization of DH5-alpha
<li>Minimal media characterization of DH5-alpha
<li>Modelling survival of PR transformed E.coli cells v/s wild type E.coli
<li>Modelling survival of PR transformed E.coli cells v/s wild type E.coli
-
<li>Literature survey on increase in recombinant protein expression in PR transformed cells</br></br>
+
<li>Literature survey on increase in recombinant protein expression in PR transformed cells
<h2>Week 3</h2>
<h2>Week 3</h2>
<li>Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea.
<li>Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea.

Revision as of 20:02, 28 October 2011

bar iGEM 2011 - Home Page Indian Institute of Technology - Madras




Contents

Notebook

Week 1

  • Metabolic simulation and modelling of increase in proton flux on addition of Proteorhodopsin
  • Literature survey on expression of Proteorhodopsin in E.coli

    Week 2

  • Minimal media characterization of DH5-alpha
  • Modelling survival of PR transformed E.coli cells v/s wild type E.coli
  • Literature survey on increase in recombinant protein expression in PR transformed cells

    Week 3

  • Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea.
  • Designing the incubator for experimental studies with Proteorhodopsin
  • Experimental analysis of growth rates of PR transformed E.coli and DH5-alpha in presence of ETC-Inhibitor Azide

    Week 4

  • Standardizing the protocol for digestion of pKJ900
  • Designing RFP generator for promoter studies

    Week5

  • Generating the PcstA_RFP Generator
  • Synthesizing the T7_RFP Generator

    Week6

  • Characterizing PcstA_RFP Generator
  • Designing the promoters for Proteorhodopsin gene
  • Codon optimization of Proteorhodopsin gene

    Week7

  • Designing the OmpR Promoter_RFP Generator
  • Designing the PcstA_Luciferase Generator
  • Digestion of Promoters

    Week8

  • Transformation of Promoters
  • Standardizing the protocols for transformation of proteorhodopsin
  • Designing the Proteorhodopsin Generator

    Week9

  • Preparation for Presentation at IITM Research Expo
  • Lecture preparation on synthetic biology
  • Characterizing growth behaviour of Proteorhodopsin transformed cells on various minimal media under light/dark conditions.

    Week10

  • Expression studies of the recombinant protein in proteorhodopsin transformed E.coli
  • Presentation at IITM Research Expo

    Week11

  • Wiki designing
    • Retrieved from "http://2011.igem.org/Team:IIT_Madras/Notebook"