Team:NYMU-Taipei/results/immunological-solution1

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Revision as of 03:03, 6 October 2011 (view source) Phonesents (Talk | contribs) (→Construction & Parts) ← Older edit		Revision as of 03:10, 6 October 2011 (view source) BarryChen (Talk | contribs) (→Measurements of Construct tetR+rbs+GFP under Fluorescence Microscope) Newer edit →
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-	==<font size=5><font color=crimson>'''Measurements of Construct <i>tetR</i>+<i>rbs</i>+<i>GFP</i> under Fluorescence Microscope'''</font></font>==	+	==<font size=5><font color=crimson>'''Measurements of Construct <i>tetR</i>+<i>rbs</i>+<i>gfp</i> under Fluorescence Microscope'''</font></font>==

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Revision as of 03:10, 6 October 2011

• Home Get to HOME
• Idea Our Idea
  Concept Brainstorming Background Review
• Design/ Experiment
  Optomagnetic Design Protein Modelling NeuroSymbiosis Chassis SynthoPrime Lab Protocol
• Results
  Optomagnetic Bacteria NeuroSymbiosis Chassis Parts International Collaboration
• Human Practice & Safety
  Synthetic Neurobiology Human Practice Evaluation & Safety Assessment
• About Us
  The School The Team China Meetup Acknowledgement

Construction & Parts

Our minC Construct and Inv & LLO Construct on AMB-1

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K624053 BBa_K624053]

Fig. Design of minC Construct on AMB-1Backbone Plasmid: pYMB	Fig. Design of Inv & LLO Construct on AMB-1Backbone Plasmid: pRKm415

Symbiosis with Human Glial Cell

It is well-studied that Magnetotactic bacteria be introduced into guanulocytes and monocytes by phagocytosis.(Tadashi Matsunaga et all. 1986) In order to simulate the circumstances in brain, we put E.coli and AMB-1 separately into mixed glial cells in vitro. Below are the photos we captured under microscope.

Measurements of Construct tetR+rbs+gfp under Fluorescence Microscope

These are the measurements of our construct [http://partsregistry.org/wiki/index.php?title=Part:BBa_K624001 tetR+rbs+GFP](BBa_K624001) under fluorescence microscope. The left graph is from the control group of E. coli strain DH5-alpha. The middle is tetR+rbs+gfp in plasmid pSB1C3 in E. coli, while the right is from a plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+rbs+gfp which emits light is thought to be a leak phenomenon of plasmid, while RE digest has proved that the sequence length is correct, and further sequencing work is under operation.

gfp measurements under different excitation wavelengths

The expression of gfp is measured precisely under excitation wavelength from 475nm to 501nm, given the absorption wavelength 511nm. In our measurement, wavelength over 493nm yields high background, resulting in values of control group surging far beyond normal condition. Thus, 493nm is suggested as the best excitation wavelength, as it presents the highest emission light and the lowest of the control group.