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Team:HIT-Harbin/Notebook
From 2011.igem.org
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<p>Boil the mixture in autoclave at 115℃ for 20min</p> | <p>Boil the mixture in autoclave at 115℃ for 20min</p> | ||
<p>Range of application: Lactobacillus bulgaricus</p> | <p>Range of application: Lactobacillus bulgaricus</p> | ||
| - | <p>2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub> | + | <p>2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub></p> |
| - | <p>SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub> | + | <p>SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub></p> |
==='''Hogg-Jago glucose broth(HJG) medium'''=== | ==='''Hogg-Jago glucose broth(HJG) medium'''=== | ||
| - | + | <p>Tryptone 30g</p> | |
| + | <p>Yeast extract 10g</p> | ||
| + | <p>Beef extract 2g</p> | ||
| + | <p>KH<sub>2</sub>PO<sub>4</sub> 5g</p> | ||
| + | <p>Glucose 5g</p> | ||
| + | <p>Agar 1.5%</p> | ||
| + | <p>Distilled water 1000ml</p> | ||
| + | <p>Boil the mixture in autoclave at 115℃ for 20min</p> | ||
| + | <p>Range of application: Streptococcus thermophilus</p> | ||
| + | <p>HJGL is Hogg-Jago glucose broth supplemented with 0.5% lactose, whereas HJGLS is HJGL supplemented with 0.4M D-sorbitol</p> | ||
| + | <p>Agar plates were prepared by adding 1.5% (wt/vol) agar to the media</p> | ||
=='''Competent Cell and Electroporation'''== | =='''Competent Cell and Electroporation'''== | ||
==='''The competent cell of Lactobacillus bulgaricus'''=== | ==='''The competent cell of Lactobacillus bulgaricus'''=== | ||
Revision as of 03:04, 6 October 2011
Contents |
Weekly Diary
Protocal
The Composition Of Media
LB Medium
Yeast extact 5g
Peptone 10g
NaCl 10g
Agar 1-2%
Distilled water 1000ml pH 7.0
Boil the mixture in autoclave at 121℃ for 30min
Range of applicationb: Escherichia.coli
MRS Medium
Peptone 10g
Beef extract 10g
Yeast extact 5g
K2HPO4 2g
Tween 80 1ml
Diamine citrate 2g
Na-acetate 5g
MgSO4.7H2O 0.5g
MnSO4.H2O 0.25g
Glucose 20g
Agar 1-2%
Distilled water 1000ml Adjust pH to 6.4-6.8
Boil the mixture in autoclave at 115℃ for 20min
Range of application: Lactobacillus bulgaricus
2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl2,20mmol/L CaCl2
SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl2,20mmol/L CaCl2
Hogg-Jago glucose broth(HJG) medium
Tryptone 30g
Yeast extract 10g
Beef extract 2g
KH2PO4 5g
Glucose 5g
Agar 1.5%
Distilled water 1000ml
Boil the mixture in autoclave at 115℃ for 20min
Range of application: Streptococcus thermophilus
HJGL is Hogg-Jago glucose broth supplemented with 0.5% lactose, whereas HJGLS is HJGL supplemented with 0.4M D-sorbitol
Agar plates were prepared by adding 1.5% (wt/vol) agar to the media
Competent Cell and Electroporation
The competent cell of Lactobacillus bulgaricus
The competent cell of Streptococcus thermophilus
PCR Reaction
Agarose Gel Electrophoresis
Gel Purification of DNA (Solarbio QIAquick Gel Extraction Kit)
