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Team:HIT-Harbin/Notebook
From 2011.igem.org
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<p>Range of applicationb: Escherichia.coli</p> | <p>Range of applicationb: Escherichia.coli</p> | ||
==='''MRS Medium'''=== | ==='''MRS Medium'''=== | ||
| - | <p>Peptone 10g<p> | + | <p>Peptone 10g</p> |
| - | <p>Beef extract 10g<p> | + | <p>Beef extract 10g</p> |
| - | <p>Yeast extact 5g<p> | + | <p>Yeast extact 5g</p> |
| - | <p>K<sub>2</sub>HPO<sub>4</sub> 2g<p> | + | <p>K<sub>2</sub>HPO<sub>4</sub> 2g</p> |
| - | <p>Tween 80 1ml<p> | + | <p>Tween 80 1ml</p> |
| - | <p>Diamine citrate 2g<p> | + | <p>Diamine citrate 2g</p> |
| - | <p>Na-acetate 5g<p> | + | <p>Na-acetate 5g</p> |
| - | <p>MgSO<sub>4</sub>.7H<sub>2</sub>O 0.5g<p> | + | <p>MgSO<sub>4</sub>.7H<sub>2</sub>O 0.5g</p> |
| - | <p>MnSO<sub>4</sub>.H<sub>2</sub>O 0.25g<p> | + | <p>MnSO<sub>4</sub>.H<sub>2</sub>O 0.25g</p> |
| - | <p>Glucose 20g<p> | + | <p>Glucose 20g</p> |
| - | <p>Agar 1-2%<p> | + | <p>Agar 1-2%</p> |
| - | <p>Distilled water 1000ml Adjust pH to 6.4-6.8<p> | + | <p>Distilled water 1000ml Adjust pH to 6.4-6.8</p> |
| - | <p>Boil the mixture in autoclave at 115℃ for 20min<p> | + | <p>Boil the mixture in autoclave at 115℃ for 20min</p> |
| - | <p>Range of application: Lactobacillus bulgaricus<p> | + | <p>Range of application: Lactobacillus bulgaricus</p> |
| - | <p>2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub>.<p> | + | <p>2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub>.</p> |
| - | <p>SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub>.<p> | + | <p>SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub>.</p> |
==='''Hogg-Jago glucose broth(HJG) medium'''=== | ==='''Hogg-Jago glucose broth(HJG) medium'''=== | ||
Revision as of 03:00, 6 October 2011
Contents |
Weekly Diary
Protocal
The Composition Of Media
LB Medium
Yeast extact 5g
Peptone 10g
NaCl 10g
Agar 1-2%
Distilled water 1000ml pH 7.0
Boil the mixture in autoclave at 121℃ for 30min
Range of applicationb: Escherichia.coli
MRS Medium
Peptone 10g
Beef extract 10g
Yeast extact 5g
K2HPO4 2g
Tween 80 1ml
Diamine citrate 2g
Na-acetate 5g
MgSO4.7H2O 0.5g
MnSO4.H2O 0.25g
Glucose 20g
Agar 1-2%
Distilled water 1000ml Adjust pH to 6.4-6.8
Boil the mixture in autoclave at 115℃ for 20min
Range of application: Lactobacillus bulgaricus
2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl2,20mmol/L CaCl2.
SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl2,20mmol/L CaCl2.
Hogg-Jago glucose broth(HJG) medium
Competent Cell and Electroporation
The competent cell of Lactobacillus bulgaricus
The competent cell of Streptococcus thermophilus
PCR Reaction
Agarose Gel Electrophoresis
Gel Purification of DNA (Solarbio QIAquick Gel Extraction Kit)
