Team:SYSU-China/page project functional construction

From 2011.igem.org

(Difference between revisions)
Line 246: Line 246:
                 <li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li>
                 <li><a href="https://2011.igem.org/Team:SYSU-China/page_project_functional_construction"><span>Functional Verification</span></a></li>
                 <li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li>
                 <li><a href="https://2011.igem.org/Team:SYSU-China/page_project_notes"><span>Notes</span></a></li>
-
                 <li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Date Page</span></a></li>
+
                 <li><a href="https://2011.igem.org/Team:SYSU-China/page_project_datapage"><span>Data Page</span></a></li>
                 <li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li>
                 <li><a href="https://2011.igem.org/Team:SYSU-China/page_project_safety"><span>Safety</span></a></li>
               </ul>
               </ul>
Line 293: Line 293:
     <div id="page_content_textBar_enterTEXT">
     <div id="page_content_textBar_enterTEXT">
       <p>&nbsp;&nbsp;&nbsp;&nbsp;After the modules verification, we began further tests of our constructed bacteria to verify that the genes can function well.</p>
       <p>&nbsp;&nbsp;&nbsp;&nbsp;After the modules verification, we began further tests of our constructed bacteria to verify that the genes can function well.</p>
-
       <p>&nbsp;&nbsp;&nbsp;&nbsp;First, we tested the function of our constructed gene cheZ. Because of limited conditions, we tried to replace ion radiation with common UV light, which has the same effect of DNA damage to activate the SOS signal pathway. When exposed to a certain intensity(which couldn't be measured for a lack of apposite apparatus) of UV light, the E.coli transformed with RecA-CheZ-pET28a was observed a faster movement towards it.  </p>
+
       <p>&nbsp;&nbsp;&nbsp;&nbsp;First, we tested the function of our constructed gene cheZ.
-
      <p>&nbsp;&nbsp;&nbsp;&nbsp;Then, we used TrkD-pET32a to test the function of trkD, which could be accomplished through detection of the accumulation of Cs+ in E.coli extract. The results indicate that within the inducement of 0.5mM IPTG at 37℃ for 4h, the experimental group has a significantly higher amount of Cs+ absorption than the control group. </p>
+
-
      <p>&nbsp;&nbsp;&nbsp;&nbsp;In terms of the results above, we have proved that cheZ and trkD constructed in the plasmids functions. Taking these two evidence together, it's not hard to predict that the constructed bacteria obtain the ability to 'swim' towards ion radiation and to absorb Cs+.  </p>
+
-
    </div>
+
-
  </div>
+
-
</div>
+
-
<!---------------------------------------------!-->
+
-
<div class="page_content_RsideBar">
+
-
  <div class="page_content_videoBar">
+
-
<h1>Please watch our animination</h1>
+
-
    <iframe width="307" height="225" src="http://www.youtube.com/embed/KymdcolAhcE?rel=0" frameborder="0" allowfullscreen></iframe>
+
-
 
+
-
<br/>
+
-
 
+
-
  </div>
+
-
  <div class="page_content_relatedBar_project_fv">
+
-
    <h2>Pages we think helpful:</h2>
+
-
    <h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_construction">see how we construct our modules</a></h1>
+
-
    <h1><a href="https://2011.igem.org/Team:SYSU-China/page_project_modules_verification">see how we verify each module</a></h1>
+
-
    <h1><a href="https://2011.igem.org/Team:SYSU-China/main_page_story">or see our whole idea in Story page</a></h1>
+
-
    <p>&nbsp;</p>
+
-
</div>
+
-
</div> 
+
-
<!---------------------------------------------!-->
+
-
<div class="main_page_clearer_light"></div>
+
-
<!---------------------------------------------!-->     
+
-
     
+
-
  </div><!--here ends the page_content_WholeBox!-->
+
-
  <div class="main_page_clearer_light"></div>
+
-
  </div><!--here ends the mainpage_project_WholeContainer!-->
+
-
 
+
-
<!--here begins the contact_bottombar!-->
+
-
    <div class="contact_bottombar">
+
-
      <p>From the 2011 iGEM team SYSU-China (2011)</p>
+
-
      <p>Sun Yat-Sen University, Guangzhou, China</p>
+
-
      <p>Address: 135# Xingang Rd.(W.), Haizhu Guangzhou, P.R.China</p>
+
-
      <p><a href="http://eng.sysu.edu.cn/">visit the Sun Yat-sen university website</a></p>
+
-
      <p>Thanks Apycom jQuery Menus and <a href="http://apycom.com/">visit their website</a>
+
-
  </div>
+
-
    <!--here ends the contact_bottombar!-->
+
-
 
+
-
</div><!--here ends the backgroud_shadow_bar!-->
+
-
</body>
+
-
</html>
+

Revision as of 03:42, 6 October 2011


Functional Verification-Sun Yat-sen Univ.

See Details

    After the modules verification, we began further tests of our constructed bacteria to verify that the genes can function well.

    First, we tested the function of our constructed gene cheZ.

Retrieved from "http://2011.igem.org/Team:SYSU-China/page_project_functional_construction"