Team:NYMU-Taipei/results/immunological-solution1

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(Difference between revisions)

Revision as of 01:32, 6 October 2011 (view source) Yuying (Talk | contribs) (→Construction & Parts) ← Older edit		Revision as of 01:38, 6 October 2011 (view source) BarryChen (Talk | contribs) Newer edit →
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-	<font size=4><font color=green>'''Our MinC construct and Inv&LLO construct'''</font></font>	+	<font size=4><font color=green>'''Our <i>minC</i> construct and <i>Inv</i> & <i>LLO</i> construct'''</font></font>
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	!  [[Image: AMB-1_Inv_LLO.jpg|center]]		!  [[Image: AMB-1_Inv_LLO.jpg|center]]
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-	|  <center>Fig. Design of MinC Construct on AMB-1</center><center>Backbone Plasmid: pYMB</center>	+	|  <center>Fig. Design of <i>minC</i> Construct on AMB-1</center><center>Backbone Plasmid: pYMB</center>
-	|  <center>Fig. Design of Inv&LLO Construct on AMB-1</center><center>Backbone Plasmid: pRKM415</center>	+	|  <center>Fig. Design of <i>Inv</i> & <i>LLO</i> Construct on AMB-1</center><center>Backbone Plasmid: pRKm415</center>
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-	==<font size=5><font color=crimson>'''Symbiosis with human glial cell '''</font></font>==	+	==<font size=5><font color=crimson>'''Symbiosis with Human Glial Cell '''</font></font>==
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-	==<font size=4><font color=crimson>'''tetR+RBS+GFP '''</font></font>==	+	==<font size=4><font color=crimson>'''<i>tetR</i>+<i>rbs</i>+<i>gfp</i> '''</font></font>==
-	<font size=4>'''measurements of construct tetR+RBS+GFP under fluorescence microscope'''	+	<font size=4>'''Measurements of Construct <i>tetR</i>+<i>rbs</i>+<i>gfp</i> under Fluorescence Microscope'''
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-	<font size=3>These are the measurements of our construct tetR+RBS+GFP under fluorescence microscope. The left graph is from the control group of ''E. coli'' strain DH5a. The middle is tetR+RBS+GFP in plasmid c3 in ''E. coli'', while the right is from a plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proven the sequence length is correct, and further sequencing work is under operation.	+	<font size=3>These are the measurements of our construct <i>tetR</i>+<i>rbs</i>+<i>gfp</i> under fluorescence microscope. The left graph is from the control group of ''<i>E. coli</i>'' strain DH5a. The middle is <i>tetR</i>+<i>rbs</i>+<i>gfp</i> in plasmid pSB1C3 in ''<i>E. coli</i>'', while the right is from a plate without any bacilli. All graphs are measured in fixed exposure time. Construct of <i>tetR</i>+<i>rbs</i>+<i>gfp</i> which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proven the sequence length is correct, and further sequencing work is under operation.
-	'''GFP measurements under different excitation wavelengths'''	+	'''<i>gfp</i> measurements under different excitation wavelengths'''

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Revision as of 01:38, 6 October 2011

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• Human Practice & Safety
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• About Us
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Construction & Parts

Our minC construct and Inv & LLO construct

Fig. Design of minC Construct on AMB-1Backbone Plasmid: pYMB	Fig. Design of Inv & LLO Construct on AMB-1Backbone Plasmid: pRKm415

Symbiosis with Human Glial Cell

tetR+rbs+gfp

Measurements of Construct tetR+rbs+gfp under Fluorescence Microscope

These are the measurements of our construct tetR+rbs+gfp under fluorescence microscope. The left graph is from the control group of E. coli strain DH5a. The middle is tetR+rbs+gfp in plasmid pSB1C3 in E. coli, while the right is from a plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+rbs+gfp which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proven the sequence length is correct, and further sequencing work is under operation.

gfp measurements under different excitation wavelengths

The expression of GFP is measured precisely under excitation wavelength from 475nm to 501nm, given the absorption wavelength 511nm. In our measurement, wavelength over 493nm yields high background, resulting in values of control group surging far beyond normal condition. Thus, 493nm is suggested as the best excitation wavelength, as it presents the highest emission light and the lowest of the control group.