Team:Peking R/Notebook/ZZR

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Latest revision as of 15:25, 19 October 2011

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Zhang Zhenrun's Notebook

 

 

I participate in designing the project and detailing the experiments. Particularly I'm in charge of constructing vio operon parts containing ribozymes so that we can enhance the product purity of the metabolic pathway.

 

  
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Contents:

07.21-07.28 
Construted Terminator-pBAD promoter for part assembly in the next step.

07.29-08.05 
Attended iGEM 2011 China Meetup and had a few days’ holiday.

08.06-08.13 
Characterize deoxyviolacein primarily by TLC (developed by dichloromethane: methanol = 6:1 (v/v)) and spectrophotometry (absorbance wavelength=570nm).
Constructed Terminator(B0015)-pBAD-vioABD.

08.14-08.18
Constructed Terminator(B0015)-pBAD-vioC with scores of base pair from vioB at 5’ and scores of base pair from vioE, termed “vioCC”. Sequencing results showed that it is actually Terminator(B0015)-pBAD-vioD with scores of base pair from vioB at 5’ and scores of base pair from vioE, termed “vioDD”.
Constructed Terminator(B0015)-pBAD-vioE.

08.19-08.25
Constructed Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioE & Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE, both of whose ribosomal binding sites were mutated to AGGAGGT.

08.26-08.31
Constructed Terminator(B0015)-pBAD-vioABDE.

09.01-09.08
Constructed Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE, whose ribosomal binding site is native.

09.09-09.15
Constructed Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioD.
Constructed Terminator(B0015)-pBAD-vioABD- Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE, in the backbone of pSB3K3.

09.16-09.23
Acquired HPLC results of the extraction from E. coli producing proviolacein and deoxychromoviridans.
Constructed Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioD-Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE & Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioD -Terminator(B0015) -pBAD-vioE.

09.24-10.04
Constructed Terminator(B0015)-pBAD-vioAB- Terminator(B0015)-pBAD- TPP ribozyme 1.20-vioD- Terminator(B0015) -pBAD-vioE.
Switched the backbone of Terminator(B0015)-pBAD-vioABD- Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE to pSB1C3.
Part submission.
Wiki editing, part documentation.
 
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