Team:Peking S/extra/attribution

Handuo Shi

Concentrated on a feedback gene network, I have constructed it as well as demonstrated its effectiveness. Moreover, I helped in the modeling of our system and fulfilled an algorithm so that we can construct our boolean logic gates in the simplest way.

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Dingqiao Wen

I'm responsible for part of the molecular cloning of the chemical wires and characterizing the properties of quorum sensing repressors.

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Yiwei Chen

I took the responsibility for constructing the sRNA-dependent competitor with my partner Qin Xiao. Furthermore, our work mainly focused on the characterization of the response curve of competitor in E. coli, and finally contributed to the rational design of the competitor.

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Yanrong Ma

My task is mainly constructing and characterizing quorum sensing genes named cinI and cinR ,and further characterizing its feasibilities in constructing synthetic microbial consortia.

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Hanchi Lin

Apart from mainly assisting in the molecular clonging of the XOR gate and depicting its function, I also spend time being engaged in taking advantage of some basic biological parts to set up other Boolean synthetic gate. This summer, I am also in charge of purchasing reagent consumables. This conduces to the guarantee of our project’s going on wheels.

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Wenhan Luo

My major task is molecular cloning of generators and corresponding receivers for chemical wires such as A-factor. In addition, characterization of some logic gates like AND GATE as well as constructing a three input gate using new signaling molecules are my major contributions in the later stage of our poject. What is more, I am also responsible for the direction and manufacture of the CG animation about invertors.

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Jingchen Feng

I am mainly responsible for the modeling work of our project. I model our balancer and give simulation result of balancer and XOR gate. Besides, under the guidance of Xiao Qin and Yiwei Chen I help constructing sRNA-dependent competitor. I also participate in the microfluidic work.

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Jingyi Xi

Mainly in charge of microfluidic device, I created a bilayer microfluidic system for our project, which provides a suitable space for different cells to be spatially separated apart and population controlled, as well as allowing chemical wires to transfer freely from each side to the other. What’s more, I also took part in molecular cloning of TPP regulator, characterization of chemical wire toolbox, and modeling work of XOR gate.

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Xiao Qin

In our project, my job is mainly to construct the competitor module, as well as characterize its performance. I also have a partner, Chen Yiwei, to fulfill this task together.

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Rui Sun

I mainly focused on the construction of microbial population density balancer. In addition, I also carried out a matrix assay of the positive regulating signaling molecules demonstrating their orthogonality.

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Ruofan Yu

Since I'm mainly in charge of the team wiki and other chores,I don't have a specific task in our project.But I do help other teammates when they're having too many things on their to-do list.Please refer to Handuo Shi and Weiyue Ji's lab diary to get detailed information.

Linna Deng

I was in charge of construction of gene circuit in A cell, which plays an indispensable role in the balancer system of microbial consortia population. Additionally, I was responsible for the work in human practice.

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Weiyue Ji

My work mainly focuses on the construction and characterization of XOR gate using chemical wire from our toolbox and some basic logic gates like AND gate and OR gate.

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Host labs, advisor, and instructor of our team

The following persons from Peking University supported us a lot in our project:

1.Professor Qi Ouyang is our advisor, and he supported us with valued advice on our project. 2.Professor Chongren Xu and Xinqiang He provided the laboratory and equipments for our experiments. 3.Haoqian Zhang acted as the instructor of our team.