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Team:Peking S/data
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| - | # [http://partsregistry.org/Part:BBa_K581019 Main Page] - '''rhl repressible promoter-gfp-ssrA degradation tag, BBa_K581019''': We ceated the rhl repressible promoter by positioning the rhl-box between and partially overlapping consensus -35 and -10 hexamers with 18 bp between the hexamers. When C4-HSL represents and binds to RhlR proteins, RhlR will bind to its DNA binding site, the rhl box, thus to reduce the accessibility of RNA polymerases to this promoter, repressing the transcription initiation. | + | # [http://partsregistry.org/Part:BBa_K581019 Main Page] - '''rhl repressible promoter-gfp-ssrA degradation tag, BBa_K581019''': We ceated the rhl repressible promoter by positioning the rhl-box between and partially overlapping consensus -35 and -10 hexamers with 18 bp between the hexamers. When C4-HSL represents and binds to RhlR proteins, RhlR will bind to its DNA binding site, the rhl box, thus to reduce the accessibility of RNA polymerases to this promoter, repressing the transcription initiation. A GFP reporter with C-terminus fused ssrA degredation tag was used to report the transcriptional activity at the promoters. |
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Revision as of 19:04, 5 October 2011
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Data Page
Data For Our Favorite New Parts
- [http://partsregistry.org/Part:BBa_K581003 Main Page] - SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2), BBa_K581003: This is the conjugate part of ptsG2-gfp.
- [http://partsregistry.org/Part:BBa_K581004 Main Page] - ptsG2-GFP (ptsG2 5'UTR fused with gfp), BBa_K581004: This is the conjugate part of the small RNA regulator sgrS2.
- [http://partsregistry.org/Part:BBa_K581019 Main Page] - rhl repressible promoter-gfp-ssrA degradation tag, BBa_K581019: We ceated the rhl repressible promoter by positioning the rhl-box between and partially overlapping consensus -35 and -10 hexamers with 18 bp between the hexamers. When C4-HSL represents and binds to RhlR proteins, RhlR will bind to its DNA binding site, the rhl box, thus to reduce the accessibility of RNA polymerases to this promoter, repressing the transcription initiation. A GFP reporter with C-terminus fused ssrA degredation tag was used to report the transcriptional activity at the promoters.
