Team:HKU-Hong Kong/Parts

From 2011.igem.org

(Difference between revisions)

Revision as of 16:38, 5 October 2011

Sample Data Page

By expressing the tetR-HNS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the 2 tetR binding region (tetO2).Thus in our designed operator- repressor binding, the presence of 2 tetR binding sites are used to enhance the binding of tetR part of the fusion proteins. As the fusion proteins bound to tetO, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved.

Experience:

We have entered the confirmation data for the characterization of the selected constitutive promoters we have used. Of the 4 promoters we have used in the project, our team considered that J23109 or J23116 are more suitable promoters in creating the super silencer repression system.

These parts are:

[http://partsregistry.org/Part:BBa_J23103 BBa_J23103 ]

[http://partsregistry.org/Part:BBa_J23106 BBa_J23106 ]

[http://partsregistry.org/Part:BBa_J23109BBa_J23109 ]

[http://partsregistry.org/Part:BBa_J23116BBa_J23116 ]

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-By expressing the tetR:H-NS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the tetR binding region (tetO2). There are two tet-R binding sites as tet-R form a dimer as in the tet repressor- operator system. As the fusion proteins bound to tet-O, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell.  With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved.
+By expressing the tetR-HNS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the 2 tetR binding region (tetO2).Thus in our designed operator- repressor binding, the presence of 2 tetR binding sites are used to enhance the binding of tetR part of the fusion proteins. As the fusion proteins bound to tetO, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved.
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