Team:Kyoto/Material

From 2011.igem.org

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|08/23||c-glnG Reverse||ggactagtacacacaagctgtgaatcactc
|08/23||c-glnG Reverse||ggactagtacacacaagctgtgaatcactc
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|08/26||gln-promoter1||cggaattcgcggccgcttctagagattgca
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|08/26||gln-promoter2r||gaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc
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|08/26||gln-promoter3||gaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc
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|08/26||gln-promoter4r||ggactagtaaaagcgaaatctgtgccaacttttaaattgcccctaaaaggcgttatcatgcgcacc
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|08/28||cc-glnL Forward||ggaattcgcggccgcttctagaggagactgctttatggcaac
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|08/28||cc-glnG Forward||ggaattcgcggccgcttctagaggtgacgtttatgcaacga
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|09/13, 09/22||Add J23105 Forward||CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
|09/13, 09/22||Add J23105 Forward||CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc

Revision as of 18:30, 5 October 2011

Contents

Materials

Strain(Fruits Fly)

NameGenotype and/or Relevant FeaturesSource or Reference
D.melanogaster(fly)Oregon-R-CDGRC[http://kyotofly.kit.jp/cgi-bin/stocks/search_res_det.cgi?DB_NUM=1&DG_NUM=105669]

Strains(E.Coli)

NameGenotype and/or Relevant FeaturesSource or Reference
JM109 endA, recA1, gyrA96,thi,hsdR17,(rκ-,mκ+),relA1, supE44,λ-,Δ(lac-proAB),[F',traD36,proAB,laclqZΔM15] Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]
BW25113 ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514 E.coliwiki[http://ecoliwiki.net/colipedia/index.php/BW25113]

Primers

DateNameSequence
07/21S Forward (Same as SRRz Forward)gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga
07/21S ReverseCTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc
07/21SRRz ReverseCTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc
07/28ΔTMD1 Forwardcgcggcagatataatggcggt
08/11ΔTMD1 Reversegtcatgtttttctggcatcttcatgtct
08/11glnL Forwardtctagaggagactgctttatggcaac
08/11glnL Reverseactagtaggaactatcgtcatcgactac
08/11glnG Forwardtctagaggtgacgtttatgcaacga
08/11glnG Reverseactagtacacacaagctgtgaatcactc
08/19, 08/24Sam7 Forwardggttcattcgtgaccttctcgacttc
08/19, 08/24Sam7 Reverseaggcgataatggcgcacatc
08/20SRRz Forward RetrygGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg
08/20SRRz Reverse RetryCTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg
08/23, 08/24Deletion of Upstream Forwardgagcaaatccccttattgggggtaagacatg
08/23, 08/24Deletion of Upstream Reversectctagaagcggccgcgaattcc
08/23c-glnL Forwardgctctagaggagactgctttatggcaac
08/23c-glnL Reverseggactagtaggaactatcgtcatcgactac
08/23c-glnG Forwardgctctagaggtgacgtttatgcaacga
08/23c-glnG Reverseggactagtacacacaagctgtgaatcactc
08/26gln-promoter1cggaattcgcggccgcttctagagattgca
08/26gln-promoter2rgaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc
08/26gln-promoter3gaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc
08/26gln-promoter4rggactagtaaaagcgaaatctgtgccaacttttaaattgcccctaaaaggcgttatcatgcgcacc
08/28cc-glnL Forwardggaattcgcggccgcttctagaggagactgctttatggcaac
08/28cc-glnG Forwardggaattcgcggccgcttctagaggtgacgtttatgcaacga
09/13, 09/22Add J23105 ForwardCAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
09/13, 09/22Add J23105 ReverseAGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc
10/10Deletion of GFP Forward (Primer1)ccaggcatcaaataaaacgaaaggctc
10/10Deletion of GFP-DT Forward (Primer2)tactagtagcggccgctgc
10/10Deletion of GFP, GFP-DT Reverse (Primer3)ctctagtattattgatttctaccatcttctactcc
- VF2 (<partinfo>G00100</partinfo>)tgccacctgacgtctaagaa
- VF (<partinfo>G00101</partinfo>)attaccgcctttgagtgagc
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