Team:Tokyo-NoKoGen

From 2011.igem.org

(Difference between revisions)
Line 1: Line 1:
 +
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN" "http://www.w3.org/TR/html4/loose.dtd">
<html>
<html>
<head>
<head>
Line 4: Line 5:
<title>Home</title>
<title>Home</title>
<meta name="viewport" content="width = 960, minimum-scale = 0.25, maximum-scale = 1.60">
<meta name="viewport" content="width = 960, minimum-scale = 0.25, maximum-scale = 1.60">
-
<meta name="GENERATOR" content="Freeway 5 Express 5.5.2">
+
<meta name="GENERATOR" content="Freeway 5 Express 5.6.1">
<style type="text/css">
<style type="text/css">
<!--  
<!--  
Line 23: Line 24:
h1:first-child { margin-top:0px }
h1:first-child { margin-top:0px }
strong { font-weight:bold }
strong { font-weight:bold }
-
.style3 { font-family:Arial,Helvetica,sans-serif; margin-left:40px; margin-right:40px }
 
-
.style14 { color:#000; font-size:20px }
 
-
.style18 { color:#000 }
 
-
.style2 { font-size:20px }
 
-
.style10 { color:#000 }
 
.style12 { text-align:center }
.style12 { text-align:center }
.style13 { font-family:Arial,Helvetica,sans-serif; font-size:24px }
.style13 { font-family:Arial,Helvetica,sans-serif; font-size:24px }
 +
.style2 { font-size:20px }
.style17 { font-family:Arial,Helvetica,sans-serif; font-size:20px }
.style17 { font-family:Arial,Helvetica,sans-serif; font-size:20px }
.style9 { font-family:Arial,Helvetica,sans-serif; font-size:20px }
.style9 { font-family:Arial,Helvetica,sans-serif; font-size:20px }
-
.style32 { color:#000; font-family:Arial,Helvetica,sans-serif; font-size:24px; text-align:center }
+
.style42 { color:#000; font-family:Arial,Helvetica,sans-serif; font-size:24px; text-align:left }
-
.style31 { color:#000; font-family:Arial,Helvetica,sans-serif; font-size:24px; text-align:left }
+
-
.style33 { color:#000; font-size:18px }
+
-
.style15 { font-size:14px }
+
-
.style29 { font-size:14px }
+
.style36 { color:#f8fa53; font-family:Arial,Helvetica,sans-serif; font-size:50px; text-align:right }
.style36 { color:#f8fa53; font-family:Arial,Helvetica,sans-serif; font-size:50px; text-align:right }
.style34 { color:#fff; font-family:Arial,Helvetica,sans-serif; font-size:12px; text-align:right }
.style34 { color:#fff; font-family:Arial,Helvetica,sans-serif; font-size:12px; text-align:right }
 +
.style3 { font-family:Arial,Helvetica,sans-serif; margin-left:40px; margin-right:40px }
 +
.style14 { color:#000; font-size:20px }
 +
.style18 { color:#000 }
 +
.style10 { color:#000 }
 +
.style28 { color:#000; font-family:Arial,Helvetica,sans-serif; font-size:14px }
-->
-->
</style>
</style>
Line 70: Line 68:
<col width=10>
<col width=10>
<col width=2>
<col width=2>
-
<col width=34>
+
<col width=1>
 +
<col width=33>
<col width=317>
<col width=317>
<col width=27>
<col width=27>
Line 79: Line 78:
</colgroup>
</colgroup>
<tr valign=top>
<tr valign=top>
-
<td height=1 colspan=8></td>
+
<td height=1 colspan=9></td>
<td height=267 colspan=2 rowspan=6><img src="https://static.igem.org/mediawiki/2011/0/02/EcoLion2.png" border=0 width=302 height=267 alt="EcoLion" style="float:left"></td>
<td height=267 colspan=2 rowspan=6><img src="https://static.igem.org/mediawiki/2011/0/02/EcoLion2.png" border=0 width=302 height=267 alt="EcoLion" style="float:left"></td>
<td height=1></td>
<td height=1></td>
</tr>
</tr>
<tr valign=top>
<tr valign=top>
-
<td height=357 rowspan=7></td>
+
<td height=357 rowspan=9></td>
-
<td height=357 rowspan=7 bgcolor="#cdcdcd"><p class="style3">&nbsp;</p>
+
<td height=357 rowspan=9 bgcolor="#cdcdcd"><p class="style3">&nbsp;</p>
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen"><span class="style18" style="text-decoration:underline">Home</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen"><span class="style18" style="text-decoration:underline">Home</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Team"><span class="style18" style="text-decoration:underline">Team</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Team"><span class="style18" style="text-decoration:underline">Team</span></a></span></strong></p>
Line 92: Line 91:
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Parts"><span class="style18" style="text-decoration:underline">BioBricks</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/Parts"><span class="style18" style="text-decoration:underline">BioBricks</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/notebook"><span class="style18" style="text-decoration:underline">Notebook</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/notebook"><span class="style18" style="text-decoration:underline">Notebook</span></a></span></strong></p>
-
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/protocols"><span class="style18" style="text-decoration:underline">Protocols</span></a></span></strong></p>
+
<p class="style3"><strong><span class="style14"><a href="Protocols"><span class="style18" style="text-decoration:underline">Protocols</span></a></span></strong></p>
-
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/attribution"><span class="style18" style="text-decoration:underline">Attribution</span></a></span></strong></p>
+
<p class="style3"><strong><span class="style14"><a href="attribution"><span class="style18" style="text-decoration:underline">Attribution</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/safety"><span class="style18" style="text-decoration:underline">Safety</span></a></span></strong></p>
<p class="style3"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/safety"><span class="style18" style="text-decoration:underline">Safety</span></a></span></strong></p>
<p class="style3 f-lp"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/sponsors"><span class="style18" style="text-decoration:underline">Sponsors</span></a></span></strong></p>
<p class="style3 f-lp"><strong><span class="style14"><a href="https://2011.igem.org/Team:Tokyo-NoKoGen/sponsors"><span class="style18" style="text-decoration:underline">Sponsors</span></a></span></strong></p>
</td>
</td>
-
<td height=38 colspan=6></td>
+
<td height=38 colspan=7></td>
<td height=38></td>
<td height=38></td>
</tr>
</tr>
<tr valign=top>
<tr valign=top>
-
<td height=104 colspan=3></td>
+
<td height=104 colspan=4></td>
<td height=104><img src="https://static.igem.org/mediawiki/2011/e/e4/EcoLion_Title.jpg" border=0 width=317 height=104 alt="EcoLionTitle" style="float:left"></td>
<td height=104><img src="https://static.igem.org/mediawiki/2011/e/e4/EcoLion_Title.jpg" border=0 width=317 height=104 alt="EcoLionTitle" style="float:left"></td>
<td height=104 colspan=2></td>
<td height=104 colspan=2></td>
Line 107: Line 106:
</tr>
</tr>
<tr valign=top>
<tr valign=top>
-
<td height=3 colspan=6></td>
+
<td height=3 colspan=7></td>
<td height=3></td>
<td height=3></td>
</tr>
</tr>
<tr valign=top>
<tr valign=top>
<td height=40></td>
<td height=40></td>
-
<td height=40 colspan=4><p class="style12 f-lp"><em><span class="style13"><strong>E<span class="style2">. </span></strong></span><span class="style17">col</span><span class="style9">i </span></em><span class="style13"><strong>col</strong><span class="style2">lecting heavy metal </span><strong>ion</strong><span class="style2">s</span></span></p>
+
<td height=40 colspan=5><p class="style12 f-lp"><em><span class="style13"><strong>E<span class="style2">. </span></strong></span><span class="style17">col</span><span class="style9">i </span></em><span class="style13"><strong>col</strong><span class="style2">lecting heavy metal </span><strong>ion</strong><span class="style2">s</span></span></p>
</td>
</td>
<td height=40></td>
<td height=40></td>
Line 118: Line 117:
</tr>
</tr>
<tr valign=top>
<tr valign=top>
-
<td height=81 colspan=6></td>
+
<td height=81 colspan=7></td>
<td height=81></td>
<td height=81></td>
</tr>
</tr>
<tr valign=top>
<tr valign=top>
-
<td height=10 colspan=8></td>
+
<td height=10 colspan=9></td>
<td height=10></td>
<td height=10></td>
</tr>
</tr>
<tr valign=top>
<tr valign=top>
-
<td height=81 colspan=2></td>
+
<td height=29 colspan=2></td>
-
<td height=209 colspan=5 rowspan=2><p class="style21"><strong>Project abstract</strong></p>
+
<td height=29 colspan=6><p class="style42 f-lp"><strong>Project abstract</strong></p>
-
<p class="style21 f-lp"><span class="style21"><span class="style15"> We developed the EcoLion, which collects heavy metals from the environment, aimed at cleaning up heavy-metal pollution as well as for the mining of valuable metals for industrial applications. A bacterial micro-compartment (BMC) was engineered into our </span><em><span class="style15">E. coli</span></em><span class="style15"> micromachine to act as a tank to accumulate heavy metals. Heavy metal ions, such as Zn2+, Cd2+, and As2+</span></span><span class="style21">, that are taken up by the cell will be trapped inside the tank by a metallothionein fused to a BMC-localizing tag. Using BMC in such a way will be very advantageous as it may achieve the accumulation of high concentrations of heavy metals in one place. The EcoLion that has stored heavy metals will be conveniently collected by light using phototaxis or self-aggregation. This EcoLion may ultimately be applied for collecting a number of different toxic or valuable molecules by using specific target-binding peptides or proteins.</span></p>
+
</td>
</td>
-
<td height=209 rowspan=2></td>
+
<td height=29></td>
-
<td height=81></td>
+
<td height=29></td>
 +
</tr>
 +
<tr valign=top>
 +
<td height=2 colspan=9></td>
 +
<td height=2></td>
 +
</tr>
 +
<tr valign=top>
 +
<td height=50 colspan=3></td>
 +
<td height=144 colspan=5 rowspan=2><p class="f-lp"><span class="style28">We developed the EcoLion, which collects heavy metals from the environment, aimed at cleaning up heavy-metal pollution as well as for the mining of valuable metals for industrial applications. A bacterial micro-compartment (BMC) was engineered into our <em>E. coli</em> micromachine to act as a tank to accumulate heavy metals. Heavy metal ions, such as Zn2+, Cd2+, and As2+, that are taken up by the cell will be trapped inside the tank by a metallothionein fused to a BMC-localizing tag. Using BMC in such a way will be very advantageous as it may achieve the accumulation of high concentrations of heavy metals in one place. The EcoLion that has stored heavy metals will be conveniently collected by light using phototaxis or self-aggregation. This EcoLion may ultimately be applied for collecting a number of different toxic or valuable molecules by using specific target-binding peptides or proteins.</span></p>
 +
</td>
 +
<td height=144 rowspan=2></td>
 +
<td height=50></td>
</tr>
</tr>
<tr valign=top>
<tr valign=top>
-
<td height=128 colspan=4></td>
+
<td height=94 colspan=5></td>
-
<td height=128></td>
+
<td height=94></td>
</tr>
</tr>
<tr class="f-sp">
<tr class="f-sp">
Line 142: Line 151:
<td><img src="Resources/_clear.gif" border=0 width=10 height=1 alt="" style="float:left"></td>
<td><img src="Resources/_clear.gif" border=0 width=10 height=1 alt="" style="float:left"></td>
<td><img src="Resources/_clear.gif" border=0 width=2 height=1 alt="" style="float:left"></td>
<td><img src="Resources/_clear.gif" border=0 width=2 height=1 alt="" style="float:left"></td>
-
<td><img src="Resources/_clear.gif" border=0 width=34 height=1 alt="" style="float:left"></td>
+
<td><img src="Resources/_clear.gif" border=0 width=1 height=1 alt="" style="float:left"></td>
 +
<td><img src="Resources/_clear.gif" border=0 width=33 height=1 alt="" style="float:left"></td>
<td><img src="Resources/_clear.gif" border=0 width=317 height=1 alt="" style="float:left"></td>
<td><img src="Resources/_clear.gif" border=0 width=317 height=1 alt="" style="float:left"></td>
<td><img src="Resources/_clear.gif" border=0 width=27 height=1 alt="" style="float:left"></td>
<td><img src="Resources/_clear.gif" border=0 width=27 height=1 alt="" style="float:left"></td>

Revision as of 13:37, 5 October 2011

<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN" "http://www.w3.org/TR/html4/loose.dtd"> Home

Tokyo-NokoGen 2011

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
EcoLion

 

Home

Team

Members

Project: EcoLion

BioBricks

Notebook

Protocols

Attribution

Safety

Sponsors
EcoLionTitle

E. coli collecting heavy metal ions

Project abstract

We developed the EcoLion, which collects heavy metals from the environment, aimed at cleaning up heavy-metal pollution as well as for the mining of valuable metals for industrial applications. A bacterial micro-compartment (BMC) was engineered into our E. coli micromachine to act as a tank to accumulate heavy metals. Heavy metal ions, such as Zn2+, Cd2+, and As2+, that are taken up by the cell will be trapped inside the tank by a metallothionein fused to a BMC-localizing tag. Using BMC in such a way will be very advantageous as it may achieve the accumulation of high concentrations of heavy metals in one place. The EcoLion that has stored heavy metals will be conveniently collected by light using phototaxis or self-aggregation. This EcoLion may ultimately be applied for collecting a number of different toxic or valuable molecules by using specific target-binding peptides or proteins.

Retrieved from "http://2011.igem.org/Team:Tokyo-NoKoGen"