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Team:Tokyo Metropolitan/Notebook/A19
From 2011.igem.org
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Method | Method | ||
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Electrophoresis for Hns and parts. | Electrophoresis for Hns and parts. | ||
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1. Suspended 1 µl loading buffer and 2 µl sample on parafilm. | 1. Suspended 1 µl loading buffer and 2 µl sample on parafilm. | ||
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2. Put gel in the electrophoresis tank. Poured marker and samples into well. | 2. Put gel in the electrophoresis tank. Poured marker and samples into well. | ||
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3. Electrophoresed for 30 minuets at 100V. Dyed for 10 minuets by ethidium bromide (EtBr). | 3. Electrophoresed for 30 minuets at 100V. Dyed for 10 minuets by ethidium bromide (EtBr). | ||
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4. Took a phot under UV light and checked bands. | 4. Took a phot under UV light and checked bands. | ||
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Solution of parts made bands, but solution of Hns did not make bands. | Solution of parts made bands, but solution of Hns did not make bands. | ||
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| + | = -Targeting- = | ||
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| + | Diveded K124017 plasmids solution into three microcentrifuge tubes, then extracted. Kept extracts in freezer. | ||
Revision as of 09:44, 5 October 2011
-Speeding Up-
Method
Electrophoresis for Hns and parts.
1. Suspended 1 µl loading buffer and 2 µl sample on parafilm.
2. Put gel in the electrophoresis tank. Poured marker and samples into well.
3. Electrophoresed for 30 minuets at 100V. Dyed for 10 minuets by ethidium bromide (EtBr).
4. Took a phot under UV light and checked bands.
Solution of parts made bands, but solution of Hns did not make bands.
-Targeting-
Diveded K124017 plasmids solution into three microcentrifuge tubes, then extracted. Kept extracts in freezer.
