Team:Tokyo Metropolitan/Notebook/S19

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(Created page with "=Speeding Up= Member Matsumura Method Electrophoresis for Hns and parts. 1. Suspended 1 µl loading buffer and 2 µl sample on parafilm. 2. Put gel in the electrophoresis tank....")
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=Speeding Up=
 
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Member
 
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Matsumura
 
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Method
 
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Electrophoresis for Hns and parts.
 
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1. Suspended 1 µl loading buffer and 2 µl sample on parafilm.
 
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2. Put gel in the electrophoresis tank. Poured marker and samples into well.
 
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3. Electrophoresed for 30 minuets at 100V. Dyed for 10 minuets by ethidium bromide (EtBr).
 
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4. Took a phot under UV light and checked bands.
 
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Solution of parts made bands, but solution of Hns did not make bands.
 

Revision as of 09:36, 5 October 2011