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Team:Tokyo Metropolitan/Notebook/A31
From 2011.igem.org
(Difference between revisions)
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-Transformation | -Transformation | ||
Transformed DAN solution. | Transformed DAN solution. | ||
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| + | =visualization= | ||
| + | Member | ||
| + | Shimura | ||
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| + | -Plasmid extraction | ||
| + | -Treat restriction enzyme (only S) | ||
| + | -Electrophoresis | ||
| + | |||
| + | Results | ||
| + | Cleared plasmid extraction but retest to check for the protocol. | ||
Revision as of 09:54, 5 October 2011
Speeding Up
Member Watanabe
-Treat restriction enzyme HNS(wt) -> X,S I120260 -> Bln I for two hours.
-DNA refining Phenol/Chloroform preparation and ethanol precipitation
-Measurement of DNA concentration 0.04 ug/ml
-Ligation HNS(wt):I20260 (expect GFP) = 3:1
-Transformation Transformed DAN solution.
visualization
Member Shimura
-Plasmid extraction -Treat restriction enzyme (only S) -Electrophoresis
Results Cleared plasmid extraction but retest to check for the protocol.
