"
Team:HKU-Hong Kong/Description
From 2011.igem.org
(Created page with "{{:Team:HKU-Hong_Kong/Templates/Header}} {| style="width:900px;background:#000000;text-align:justify;font-family: georgia, helvetica, arial, sans-serif;color:#ffffff;margin-top:2...") |
|||
| Line 3: | Line 3: | ||
|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;"|Super Silencer | |style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;"|Super Silencer | ||
|- | |- | ||
| - | |style="width:900px;"|''' | + | |style="width:900px;"|'''Promoter''' |
| - | < | + | Our Team has planned to utilize four different constitutive promoters first. We have selected BBa_J23103, BBa_J23106, BBa_J23109 and BBa_J23116 in the constitutive promoter family. In our project, we would like to select some of the suitable promoters to fine-tune our fusion protein expression so that a better repression activity can be achieved. We have tried to characterize the selected promoters by measuring the mRFP intensity in the plasmid J61002 as a confirmation of promoter activity. The confirmation data of the promoter activity have been shown as follow and input into the Experience page of these promoters. From the experimental data for promoter in this project, we believed that BBa_J23109 and BBa_J23116 are some of the suitable promoters to be used and further investigate. |
| - | < | + | |
| + | <div ALIGN=CENTER> | ||
| + | {| style="width:254px;background:#99EE63;text-align:center;font-family: georgia, helvetica, arial, sans-serif;color:#000000;margin- top:5px;padding: 2px;" cellspacing="5"; | ||
| + | |- | ||
| + | |[[Image:Promoter.jpg|250px]] | ||
| + | |- | ||
| + | |Comparison of red fluorescence intensity among 4 different promoters | ||
| + | |} | ||
| + | </div> | ||
Revision as of 07:24, 5 October 2011
| Super Silencer | ||
| Promoter
Our Team has planned to utilize four different constitutive promoters first. We have selected BBa_J23103, BBa_J23106, BBa_J23109 and BBa_J23116 in the constitutive promoter family. In our project, we would like to select some of the suitable promoters to fine-tune our fusion protein expression so that a better repression activity can be achieved. We have tried to characterize the selected promoters by measuring the mRFP intensity in the plasmid J61002 as a confirmation of promoter activity. The confirmation data of the promoter activity have been shown as follow and input into the Experience page of these promoters. From the experimental data for promoter in this project, we believed that BBa_J23109 and BBa_J23116 are some of the suitable promoters to be used and further investigate.
|
