"
Team:Grinnell/Recipes
From 2011.igem.org
(Difference between revisions)
| Line 102: | Line 102: | ||
</table> | </table> | ||
<br/><b>Note</b>: for liquid cultures in PYE broth, use half the concentration that would be used for a PYE plate (LB remains the same for liquid and sold cultures). | <br/><b>Note</b>: for liquid cultures in PYE broth, use half the concentration that would be used for a PYE plate (LB remains the same for liquid and sold cultures). | ||
| + | |||
| + | <h2><a name="freezerStocks"></a>Freezer Stocks</h2> | ||
| + | <ul style="list-style:none;">To an appropriate tube add: | ||
| + | <li>700μL overnight liquid culture</li> | ||
| + | <li>300μL 50% glycerol sol'n</li> | ||
| + | </ul> | ||
| + | Then place in storage at -80°C. | ||
<!-- | <!-- | ||
<h2><a name=""></a></h2> | <h2><a name=""></a></h2> | ||
--> | --> | ||
| + | </html> | ||
Revision as of 20:51, 15 July 2011
Recipes
L Broth
For 1L:- 10g Tryptone
- 5g Yeast extract
- 5g NaCl
PYE Broth
For 1L:- 2g Bacto peptone
- 1g Yeast extract
- 1mL 1M MgSO4⋅7H2O
- 1mL 0.5M CaCl2
Agar Plates
Add 15g Bacto-agar to 1L of liquid media before autoclaving.Antibiotics Kitchen
| Antibiotic | Color Code | Stock (mg/mL) | LB Plate Conc (μg/mL) | PYE Plate Conc (μg/mL) |
|---|---|---|---|---|
| Ampicillin (amp) | Red | 50 | 100 | 50 |
| Carbenicillin (carb) | Red | 50 | 50 | 50 |
| Chloramphenicol (chlor) | 2 Orange | 5 (in EtOH) | 30 | 1 |
| Kanamycin (kan) | blue | 50 | 50 | 25 |
| Nalidixic acid (nal) | Green | 10 | 20 | 20 |
| Tetracycline (tet) | Black | 2 (in EtOH) | 12 | – |
| Oxytetracycline (oxytet) | Black | 2 (in EtOH) | − | 2 |
| Spectinomycin (spec) | Purple | 20 | 50 | 100 |
Note: for liquid cultures in PYE broth, use half the concentration that would be used for a PYE plate (LB remains the same for liquid and sold cultures).
Freezer Stocks
- To an appropriate tube add:
- 700μL overnight liquid culture
- 300μL 50% glycerol sol'n
