"
Team:NYMU-Taipei/chassis
From 2011.igem.org
(Difference between revisions)
(→Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...) |
(→Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...) |
||
| Line 28: | Line 28: | ||
| - | We also refered a paper which added '''Polypeptone''' , '''Yeast extract''' and '''L-cysteine''' into MSGM. Which called '''<font color=red>En-rich MSGM</font>'''.In <Effects of growth medium composition, iron sources and atmospheric oxygen concentrations on production of luciferase-bacterial magnetic particle complex by a recombinant Magnetospirillum magneticum AMB-1,2001> | + | We also refered a paper which added '''Polypeptone''' , '''Yeast extract''' and '''L-cysteine''' into MSGM. Which called '''<font color=red>En-rich MSGM</font>'''.In <<''Effects of growth medium composition, iron sources and atmospheric oxygen concentrations on production of luciferase-bacterial magnetic particle complex by a recombinant Magnetospirillum magneticum AMB-1,2001''>> |
● | ● | ||
● | ● | ||
Revision as of 15:55, 4 October 2011
Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...
Like E-coli, AMB-1 also grows in liquid medium, and this medium called Magnetic Spirillum Growth Medium (MSGM). In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.
MSGM's ingredients
(In grams per liter)
●succinic acid 0.74 g
●KH2PO4 0.68 g
●sodium nitrate 0.12 g
●sodium thioglycolate 0.1 g
●Wolfe’s vitamin solution 10 ml(stock)
●Wolfe’s mineral solution 5 ml(stock)
●ferric quinate solution 2 ml(0.27 g of FeCl3 and 0.19 g of quinic acid in 100 ml water)
We also refered a paper which added Polypeptone , Yeast extract and L-cysteine into MSGM. Which called En-rich MSGM.In <<Effects of growth medium composition, iron sources and atmospheric oxygen concentrations on production of luciferase-bacterial magnetic particle complex by a recombinant Magnetospirillum magneticum AMB-1,2001>>
● ●
