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Team:NYMU-Taipei/chassis
From 2011.igem.org
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(→Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...) |
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<font color=red>(In grams per liter)</font> | <font color=red>(In grams per liter)</font> | ||
| - | ●succinic acid 0.74 g | + | ●succinic acid 0.74 g |
| - | ●KH2PO4 0.68 g | + | ●KH2PO4 0.68 g |
| - | ●sodium nitrate 0.12 g | + | ●sodium nitrate 0.12 g |
| - | ●sodium thioglycolate 0.1 g | + | ●sodium thioglycolate 0.1 g |
| - | ●Wolfe’s vitamin solution 10 ml(stock) | + | ●Wolfe’s vitamin solution 10 ml(stock) |
| - | ●Wolfe’s mineral solution 5 ml(stock) | + | ●Wolfe’s mineral solution 5 ml(stock) |
| - | ●ferric quinate solution 2 ml(0.27 g of FeCl<font size=1>3</font> and 0.19 g of quinic acid in 100 ml water) | + | ●ferric quinate solution 2 ml(0.27 g of FeCl<font size=1>3</font> and 0.19 g of quinic acid in 100 ml water) |
Revision as of 15:47, 4 October 2011
Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...
Like E-coli, AMB-1 also grows in liquid medium, and this medium called Magnetic Spirillum Growth Medium (MSGM). In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.
MSGM's ingredients
(In grams per liter)
●succinic acid 0.74 g
●KH2PO4 0.68 g
●sodium nitrate 0.12 g
●sodium thioglycolate 0.1 g
●Wolfe’s vitamin solution 10 ml(stock)
●Wolfe’s mineral solution 5 ml(stock)
●ferric quinate solution 2 ml(0.27 g of FeCl3 and 0.19 g of quinic acid in 100 ml water)
We also refered a paper which added Polypeptone and Yeast extract into MSGM. Which called En-rich MSGM
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