Team:WHU-China/Project

    Stepping into the IGEM world for the first time, we studied a huge amount of previous projects and found that light sensing bacteria kept arousing general interest but remain an unsettled issue. Despite the great efforts made by previous teams, little progress was made in this field. Therefore we decide to make some attempts. We focus on constructing colorful E.coli.
    Firstly, we came up with the idea of “colorful E.film” based on previous research of bio-imaging. The greatest advantage of our “E.film” against traditional chemical film is the extremely small size of pixels. And we hope to create a colorful film instead of a black-and-white one by constructing three E.coli strains which can produce and secrete three primary colors respectively in the presence of different lights.......

    This summer we designed two projects: color film and oscillator. One is about time and another is about space. The oscillator is to construct one strain colorful E.coli changing color periodically. The color film project is to construct three strains of E.coli which can form a colorful image. Here is the detailed design of these two projects.

As the changes of cycle time with the change of the parameters in the image , we can get the conclusion that:
     1.have little influence on the range.
     2.do not have much influence on the cycle time.
     3.have inversely proportional relationship with the cycle time , it have a huge influence on the cycle time , but little impact on the range (Can be used to adjust the cycle to maintain constant amplitude)
     4.When, have little influence on the range.
This conclusion we get before can be used to guide the design of biological systems, in order to achieve the adjustment period, amplitude can adjust the size of some parameters.........

   Blue light induced bacteria with part BBa_K560000, blue light sensing and reporting system. E.coli cultures from the same clone were grown in the dark under the blue light. Control were under the dark. Bacteria under blue light expressed little RFP and the bacterial colony did not turn red. Control bacterial colony turned red obviously.....