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Team:HKUST-Hong Kong/content.html
From 2011.igem.org
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| - | <b> | + | <b>Greeting from the <b>HKUST iGEM Team for 2011</b>!</b><br> |
| - | Welcome to | + | Welcome to our Wiki page!<br><br> |
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This is the fourth year for HKUST to<br> participate in this international<br> synthetic biology competition.<br><br> | This is the fourth year for HKUST to<br> participate in this international<br> synthetic biology competition.<br><br> | ||
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| - | Thanks to our experienced instructors, considerate advisors and <br>every team member | + | Thanks to our experienced instructors, considerate advisors and <br> effort from every team member of the HKUST iGEM 2011 team, we enjoyed<br> a fantastic summer working together to achieve our goal. <br><br> |
| - | You may visit our <a href=gallery.html target=_top><font color=white><u>Gallery</u></font></a> to see | + | You may visit our <a href=gallery.html target=_top><font color=white><u>Gallery</u></font></a> to see what we do in the lab. |
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| - | <font color=#CDD2C2>It has often been assumed that when an antibiotic is introduced to a bacterial community, only cells that carry resistance genes will survive and proliferate. However, recent findings | + | <font color=#CDD2C2>It has often been assumed that when an antibiotic is introduced to a bacterial community, only cells that carry resistance genes will survive and proliferate. However, recent findings suggests that communities with a mixture of highly resistant (HR) and less resistant (LR) individuals are able to survive through ‘charity’ by HR individuals, which support LR individuals through indole signalling.</p><br> |
| - | <p>Our team aims to interfere with this signalling through introducing a disruptor <i>E. coli</i> into the bacterial community. This new strain will be able to degrade indole using a mutated toluene-4-monooxygenase (T4MO). We hypothesize that LR cells in the community deprived of indole will undergo | + | <p>Our team aims to interfere with this signalling through introducing a disruptor <i>E. coli</i> into the bacterial community. This new strain will be able to degrade indole using a mutated toluene-4-monooxygenase (T4MO). We hypothesize that LR cells in the community deprived of indole will undergo elimination at lower antibiotic concentrations. If this demonstration is successful, indole degradation might prove to be a possible strategy in boosting antibiotics effectiveness in medical practice against bacteria that rely on such signalling.</p><br> |
<p>Along the way, we will also create a new strain of <i>E. coli</i> that utilizes an essential gene (<i>nadE</i>) as the selection marker for transformation, allowing antibiotics-free transformation and plasmid maintenance for regular laboratory manipulation. This new transformation method can be adopted for future iGEM teams, reducing their use of antibiotics without increasing the complexity of the transformation protocol.</p><br> | <p>Along the way, we will also create a new strain of <i>E. coli</i> that utilizes an essential gene (<i>nadE</i>) as the selection marker for transformation, allowing antibiotics-free transformation and plasmid maintenance for regular laboratory manipulation. This new transformation method can be adopted for future iGEM teams, reducing their use of antibiotics without increasing the complexity of the transformation protocol.</p><br> | ||
Revision as of 05:46, 5 October 2011
Greeting from the HKUST iGEM Team for 2011! |
Overview |
Data Page |
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Acknowledgements |
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Project Abstract It has often been assumed that when an antibiotic is introduced to a bacterial community, only cells that carry resistance genes will survive and proliferate. However, recent findings suggests that communities with a mixture of highly resistant (HR) and less resistant (LR) individuals are able to survive through ‘charity’ by HR individuals, which support LR individuals through indole signalling. Our team aims to interfere with this signalling through introducing a disruptor E. coli into the bacterial community. This new strain will be able to degrade indole using a mutated toluene-4-monooxygenase (T4MO). We hypothesize that LR cells in the community deprived of indole will undergo elimination at lower antibiotic concentrations. If this demonstration is successful, indole degradation might prove to be a possible strategy in boosting antibiotics effectiveness in medical practice against bacteria that rely on such signalling. Along the way, we will also create a new strain of E. coli that utilizes an essential gene (nadE) as the selection marker for transformation, allowing antibiotics-free transformation and plasmid maintenance for regular laboratory manipulation. This new transformation method can be adopted for future iGEM teams, reducing their use of antibiotics without increasing the complexity of the transformation protocol. |
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