Team:UQ-Australia/Parts

From 2011.igem.org

(Difference between revisions)

Revision as of 09:11, 4 October 2011

The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success. Here we demonstrate a biological clock being standardised as a set of BioBrick parts.
Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.

Parts

Students

	Plac/ara A multi-domain promoter that is self-inducible by arabinose. Gene expression regulated by LacI binding to special operator sites in the promoter		glnG
	araC Encodes for a positive regulatory protein for L-arabinose utilization in E.coli		pBAD
	LacI DNA-binding transcriptional inhibitor for lac-operon		GlnAp2

New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.

<groupparts>iGEM010 UQ-Australia</groupparts>

@@ Line 23: / Line 23: @@
 |width="125"|
 |width="321"|'''<span style="color:#D4A017">Plac/ara</span>'''
-Third year Bachelor of Biomedical Sciences majoring in Neurobiology and Developmental Science
+A multi-domain promoter that is self-inducible by arabinose. Gene expression regulated by LacI binding to special operator sites in the promoter
 |width="125"|
 |width="321"|'''<span style="color:#D4A017">glnG</span>'''
-Third year Bachelor of Biotechnology majoring in Bioinformatics
 |-
 |
 |'''<span style="color:#D4A017">araC</span>'''
-Third year Bachelor of Science majoring in Physics
+Encodes for a positive regulatory protein for L-arabinose utilization in E.coli
 |
 |'''<span style="color:#D4A017">pBAD</span>'''
-Third year Bachelor of Biotechnology majoring in Molecular Biotechnology
 |-
 |
 |'''<span style="color:#D4A017">LacI</span>'''
-Third year Bachelor of Biomedical Sciences majoring in Neuroscience
+DNA-binding transcriptional inhibitor for lac-operon
 |
 |'''<span style="color:#D4A017">GlnAp2</span>'''
-Third year Bachelor of Biomedical Sciences majoring in Human Genetics and Biomedical Science
 |-
 |