Team:NYMU-Taipei/synthoprime

From 2011.igem.org

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<!--- primerone --->
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{|
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[[File:SynthoPrime manual p1.png|thumb|300px| '''STEP 1:''' Offer your sequence to be ‘biobricked’ by direct pasting your DNA or specifying by names of organism, replicon and gene.]]
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[[File:SynthoPrime manual p2.png|thumb|300px| '''SETP 2:''' Give your design constraints for primers, including length, melting temperature, GC-content of each primer and differences of Tm between forward and reverse primers. The primer selection process is powered by primer3.]]
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[[File:SynthoPrime manual p3.png|thumb|300px| '''STEP 3:''' In addition, you can choose to relax design constraints to gain more primer candidates. Besides, you can decide to put prefix or suffix or not by ‘Build Biobrick’ checkbox and specify extra bases before prefix and suffix for good enzyme digestion!]]
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[[File:SynthoPrime manual p4.png|thumb|300px| '''STEP 4:''' Click “SEND” when everything is ready or click “RESET” to set all parameters as default.]]
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== HOWTO: Output Reading ==
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[[File:SynthoPrime manual p5.png|thumb|630px|Main output page]]
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[[File:SynthoPrime manual p6.png|thumb|630px|Additional Info.]]
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== References and Feedback ==
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[[File:SynthoPrime manual p7.png|thumb|630px| For more details, please see the References or email to blent@gel.ym.edu.tw.]]
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Revision as of 09:09, 4 October 2011

Horizontal Slide Menu


STEP 1: Offer your sequence to be ‘biobricked’ by direct pasting your DNA or specifying by names of organism, replicon and gene.
SETP 2: Give your design constraints for primers, including length, melting temperature, GC-content of each primer and differences of Tm between forward and reverse primers. The primer selection process is powered by primer3.
STEP 3: In addition, you can choose to relax design constraints to gain more primer candidates. Besides, you can decide to put prefix or suffix or not by ‘Build Biobrick’ checkbox and specify extra bases before prefix and suffix for good enzyme digestion!
STEP 4: Click “SEND” when everything is ready or click “RESET” to set all parameters as default.

HOWTO: Output Reading

Main output page
Additional Info.

References and Feedback

For more details, please see the References or email to blent@gel.ym.edu.tw.
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