Team:Kyoto/Material

From 2011.igem.org

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<td>Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]
<td>Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]
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<td>BW25113</td><td>∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514</td><td>Promega</td>
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!Name||Genotype and/or Relevant Features||Source or Reference
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|TOP10||F-, ''mcr''A, &Delta;(''mrr''-''hsd''RMS-''mcr''BC), &phi;80''lac''Z&Delta;M15, &Delta;''lac''X74, ''nup''G, ''rec''A1, ''ara''D139, &Delta;(''ara''-''leu'')7697, ''gal''E15, ''gal''K16 ''rps''L(Str<sup>R</sup>), ''end''A1, &lambda;-||Invitrogen
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|rowspan="2"|KRX||rowspan="2"|[F&prime;, ''tra''D36, &Delta;''omp''P, ''pro''A<sup>+</sup>B<sup>+</sup>, ''lac''I<sup>q</sup>, &Delta;(''lac''Z)M15] &Delta;''omp''T, ''end''A1, ''rec''A1, ''gyr''A96 (Nal<sup>r</sup>), ''thi''-1, ''hsd''R17 (r<sub>k</sub><sup>–</sup>, m<sub>k</sub><sup>+</sup>), e14– (McrA–), ''rel''A1, ''sup''E44, &Delta;(''lac''-''pro''AB), &Delta;(''rha''BAD)::T7 RNA polymerase.||Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University
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|(Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698])
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|BL21(DE3)pLysS||F–, ''omp''T, ''hsd''S<sub>B</sub> (r<sub>B</sub><sup>–</sup>, m<sub>B</sub><sup>–</sup>), ''dcm'', ''gal'', &lambda;(DE3), pLysS, Cm<sup>r</sup>.||Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005]
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==Primers==
==Primers==

Revision as of 08:04, 4 October 2011

Contents

Materials

Strain(Fruits Fly)

NameGenotype and/or Relevant FeaturesSource or Reference
D.melanogaster(fly)Oregon-R-CDGRC[http://kyotofly.kit.jp/cgi-bin/stocks/search_res_det.cgi?DB_NUM=1&DG_NUM=105669]

Strains(E.Coli)

Primers

NameGenotype and/or Relevant FeaturesSource or Reference
JM109endA, recA1, gyrA96,thi,hsdR17,(rκ-,mκ+),relA1, supE44,

λ-,Δ(lac-proAB),[F',traD36,proAB,laclqZΔM15]

Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]
BW25113∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514Promega
DateNameSequence
07/21S Forward (Same as SRRz Forward)gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga
07/21S ReverseCTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc
07/21SRRz ReverseCTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc
07/28ΔTMD1 Forwardcgcggcagatataatggcggt
07/28ΔTMD1 Reversegtcatgtttttctggcatcttcatgtct
08/19, 08/24Sam7 Forwardggttcattcgtgaccttctcgacttc
08/19, 08/24Sam7 Reverseaggcgataatggcgcacatc
08/20SRRz Forward RetrygGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg
08/20SRRz Reverse RetryCTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg
08/23, 08/24Deletion of Upstream Forwardgagcaaatccccttattgggggtaagacatg
08/23, 08/24Deletion of Upstream Reversectctagaagcggccgcgaattcc
09/13, 09/22Add J23105 ForwardCAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
09/13, 09/22Add J23105 ReverseAGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc
10/10Deletion of GFP Forward (Primer1)ccaggcatcaaataaaacgaaaggctc
10/10Deletion of GFP-DT Forward (Primer2)tactagtagcggccgctgc
10/10Deletion of GFP, GFP-DT Reverse (Primer3)ctctagtattattgatttctaccatcttctactcc
- VF2 (<partinfo>G00100</partinfo>)tgccacctgacgtctaagaa
- VF (<partinfo>G00101</partinfo>)attaccgcctttgagtgagc
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