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Team:IIT Madras/Notebook/Protocols
From 2011.igem.org
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Ligation">Ligation</a></li> | <li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Ligation">Ligation</a></li> | ||
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Revision as of 16:51, 3 October 2011
Protocols
- Preperation of competent DH5alpha cells
- Transformation
- Miniprep using alkaline lysis buffers
- Agarose Gel Electrohoresis
- Restriction digestion
- Gel Elusion
- PCR
- PCR_purification
- Ligation
