Team:uOttawa/Project

From 2011.igem.org

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== '''Overall project''' ==
== '''Overall project''' ==
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Currently biobricks are often assembled and tested in a bacterial chassis however there remains a shortage of biobricks optimized to work in eukaryotes. To this end, we aim to build several biobricks optimized to work in the model organism ''S. cerevisiae'' as well as build a novel yeast chassis capable of robust characterization of these biobricks. In addition, we are developing a new assembly standard that will greatly improve upon the existing standards for biobrick assembly and allow for rapid integration into ''S. cerevisiae''.
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One of the major goals of synthetic biology is to apply engineering principles analogous to traditional engineering disciplines to genetic engineering. A way in which this is achieved is through the use of biobricks that allow for more rapid engineering of synthetic gene networks. These biobricks are often assembled and tested in a bacterial chassis however there remains a shortage of these biobricks optimized to work in eukaryotes. To this end, we are building several biobricks optimized to work in the model organism ''S. cerevisiae'' - a simple eukaryote as well as building a novel yeast chassis that will allow for the robust characterization of these biobricks. In addition, we are developing a new protocol that will greatly improve upon the existing assembly protocol for biobricks and allow for rapid integration into ''S. cerevisiae''.
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== Project Details==
== Project Details==

Revision as of 18:15, 8 July 2011


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Contents

Overall project

Currently biobricks are often assembled and tested in a bacterial chassis however there remains a shortage of biobricks optimized to work in eukaryotes. To this end, we aim to build several biobricks optimized to work in the model organism S. cerevisiae as well as build a novel yeast chassis capable of robust characterization of these biobricks. In addition, we are developing a new assembly standard that will greatly improve upon the existing standards for biobrick assembly and allow for rapid integration into S. cerevisiae.

Project Details

Part 2

The Experiments

Part 3

Results