|
|
| Line 96: |
Line 96: |
| | | | |
| | </div> | | </div> |
| - | <h4>Protocol</h4> | + | |
| - | <div class="pane"><a href="https://2011.igem.org/Team:ZJU-China/Protocol">>>Click to see our lab protocol about biofilm</a></div>
| + | |
| | </div> | | </div> |
| | | | |
| Line 124: |
Line 123: |
| | | | |
| | <div class="bigblock"> | | <div class="bigblock"> |
| - | <div class="block" id="nsheet"> | + | <div class="block" id="nsheet"> |
| - | <h3>Week9</h3><hr/> | + | <h3>Week1</h3><hr/> |
| - | | + | |
| - | <table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1"> | + | <table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1"> |
| - | <tr><td width="76">Day</td><td width="349">Note</td></tr> | + | <tr><td width="76">Day</td><td width="349">Note</td></tr> |
| | | | |
| - | <tr> | + | <tr> |
| - | <td id="sheetleft">Aug.29th | + | <td id="sheetleft">Jul.4th Monday</td> |
| - | Monday | + | <td><table id="intable" width="328" border="0" cellspacing="0" cellpadding="1"> |
| - | | + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | </td> | + | |
| - | <td><table id="intable" width="603" border="0" cellspacing="0" cellpadding="1"> | + | |
| | | | |
| - | <td width="191">• Meeting. Summarize all the experiment work so far. | + | <td width="128">• Aerobic cultivation of DH5α</td> |
| - | </td> | + | |
| | | | |
| | | | |
| - | <td width="249" >• Cut: 22M(X+P), 1C3(E+P) | + | <td width="196">• Preparation of apparatus for the formation of biofilm</td> |
| - | </td> | + | |
| - | | + | |
| | | | |
| - | </table></td> | + | </table></td> |
| - | </tr> | + | </tr> |
| - | <tr> | + | <tr> |
| - | <td id="sheetleft">Aug.30th | + | <td id="sheetleft">Jul 5th Tuesday |
| - | Tuesday | + | </td> |
| - | | + | <td> </td> |
| - | | + | </tr> |
| - | | + | <tr> |
| - | | + | <td id="sheetleft">Jul.6th |
| - | | + | |
| - | </td> | + | |
| - | <td><table id="intable" width="615" border="0" cellspacing="0" cellpadding="1"> | + | |
| - |
| + | |
| - |
| + | |
| - | | + | |
| - | </table></td>
| + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td id="sheetleft">Aug.31st | + | |
| | Wednesday | | Wednesday |
| - | | + | </td> |
| - | | + | <td><table width="217" border="0" cellspacing="0" cellpadding="1"> |
| - | | + | <tr> |
| - | | + | <td width="215">•Receiving primers ordered previously</td> |
| - | | + | |
| - | </td> | + | </tr> |
| - | <td><table width="640" border="0" cellspacing="0" cellpadding="1"> | + | </table> |
| - | <tr> | + | </td> |
| - | <td>• Cut vgb+YFP+tetR, fdhF+RFP+tetR.<br/>• Run the digestion results</td>
| + | </tr> |
| - | <td>• Culture the positive results</td>
| + | <tr> |
| - | </tr> | + | <td id="sheetleft">Jul.7th |
| - | </table> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td id="sheetleft">Sept.1st | + | |
| | Thursday | | Thursday |
| - | | + | </td> |
| - | | + | <td><table width="238" border="0" cellspacing="0" cellpadding="1"> |
| - | | + | <tr> |
| - | | + | <td width="200">• Preparation of the aliquot of the primers</td> |
| - | | + | <td width="200">• Something wrong with a shaking incubator</td> |
| - | </td> | + | </tr> |
| - | <td><table width="618" border="0" cellspacing="0" cellpadding="1"> | + | </table> |
| - | <tr> | + | </td> |
| - |
| + | </tr> |
| - | <td>• Miniprep: Y#4, R#2/3/4/5<br/> | + | <tr> |
| - | • Cut Y#4, R#2/3/4/5
| + | <td id="sheetleft">Jul.8th |
| - | </td>
| + | |
| - | <td>•Run the gel.<br/>
| + | |
| - | • Send R#3,Y#4 sample to sequencing
| + | |
| - | </td> | + | |
| - | <td>• Hypoxia culture: RFP3, RFP4<br/> | + | |
| - | • Preserve Y#4, R#2/3/4/5
| + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | </table> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td id="sheetleft">Sept.2nd | + | |
| | Friday | | Friday |
| - | | + | </td> |
| - | | + | <td><table width="222" border="0" cellspacing="0" cellpadding="1"> |
| - | | + | <tr> |
| - | </td> | + | <td width="155">• Preparation of culture plates for the transformations</td> |
| - | <td><table width="627" border="0" cellspacing="0" cellpadding="1"> | + | </tr> |
| - | <tr> | + | </table></td> |
| - | <td >• Phusion PCR</td>
| + | </tr> |
| - | <td>• Hypoxia Culture: R, Y<br/>
| + | <tr> |
| - | • Run the PCR results. Bands are confirmed right.
| + | <td id="sheetleft">Jul.9th |
| - | </td>
| + | |
| - | <td>• Purification: vgb, YFP, tetR.</td>
| + | |
| - | </tr> | + | |
| - | </table></td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td id="sheetleft">Sept.3rd | + | |
| | Saturday | | Saturday |
| - | | + | </td> |
| - | | + | <td><table width="313" border="0" cellspacing="0" cellpadding="1"> |
| - | | + | <tr> |
| - | </td> | + | <td width="127">• Preparation of culture plates for the transformations</td> |
| - | <td><table width="620" border="0" cellspacing="0" cellpadding="1"> | + | <td width="182">• protocols of transformation</td> |
| - | <tr> | + | </tr> |
| - | <td>• Culture: YFP, RFP, A25<br/> | + | </table> |
| - | • Culture in micro-oxygen: YFP, RFP, A25
| + | </td> |
| - | </td>
| + | </tr> |
| - | <td>• Culture in slope medium: RFP, YFP, A25<br/>
| + | <tr> |
| - | • Hypoxia culture: RFP, YFP, A25
| + | <td id="sheetleft">Jul.10th |
| - | </td> | + | |
| - | <td>• Check the fluorescence.<br/> | + | |
| - | • Neither RFP in hypoxia or oxygen condition.
| + | |
| - | Little YFP both in hypoxia and oxygen.
| + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | </table> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | <tr> | + | |
| - | <td id="sheetleft">Sept.4th | + | |
| | Sunday | | Sunday |
| - | | + | </td> |
| - | | + | <td><table width="246" border="0" cellspacing="0" cellpadding="1"> |
| - | </td> | + | <tr> |
| - | <td><table width="620" border="0" cellspacing="0" cellpadding="1"> | + | <td>• Several colonies were picked up and cultivated in 5mL LB medium. |
| - | <tr> | + | •Cryosectioning of biofilm |
| - | <td width="244">• Miniprep: RFP3, YFP4 | + | </td> |
| - | | + | </tr> |
| - | </td> | + | </table></td> |
| - | <td width="164">• Cut: RFP3, YFP4, !C3.
| + | </tr> |
| - | </td>
| + | |
| - | | + | </table> |
| - | </tr> | + | |
| - | </table></td> | + | |
| - | </tr> | + | |
| - | | + | |
| - | </table> | + | |
| | <p> </p> | | <p> </p> |
| - | </div>
| |
| - |
| |
| - | <div class="block" id="nsheet">
| |
| - | <h3>Week10</h3><hr/>
| |
| - | <table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1">
| |
| - | <tr><td width="76">Day</td><td width="349">Note</td></tr>
| |
| - |
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.5th
| |
| - | Monday
| |
| - |
| |
| - | </td>
| |
| - | <td><table id="intable" width="603" border="0" cellspacing="0" cellpadding="1">
| |
| - |
| |
| - | <td width="191">• Run the gel: Yu’s PCR results, 1C3, RFP3, YFP4<br/>
| |
| - | • Purification: RFP3, YFP4
| |
| - |
| |
| - | </td>
| |
| - |
| |
| - |
| |
| - | <td >• Ligation: RFP3+1C3, YFP4+1C3<br/>
| |
| - | • Hypoxia culture: RFP3, YFP4, A25.
| |
| - |
| |
| - | </td>
| |
| - | <td>• Transform the ligation results.<br/>
| |
| - | • Confirmed RFP expression only in hypoxia condition.
| |
| - | </td>
| |
| - |
| |
| - | </table></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.6th
| |
| - | Tuesday
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table id="intable" width="615" border="0" cellspacing="0" cellpadding="1">
| |
| - | <td>• Check the RFP, YFP plates.</td>
| |
| - | <td>• Colony PCR</td>
| |
| - |
| |
| - | </table></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.7th
| |
| - | Wednesday
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="640" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - | <td>• Culture YFP1/2, RFP3/4</td>
| |
| - |
| |
| - | </tr>
| |
| - | </table>
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.8th
| |
| - | Thurday
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="618" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - |
| |
| - | <td>• Preserve the strain of RFP1, YFP1/2 in 1C3.<br/>
| |
| - | • Miniprep the remains.
| |
| - |
| |
| - | </td>
| |
| - | <td>• Cut: YFP1/2, RFP1
| |
| - | </td>
| |
| - | <td>• Run the digestion results.<br/>
| |
| - | • Culture RFP2
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.9th
| |
| - | Friday
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="627" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - | <td >• Miniprep: RFP2, YFP2, RFP1</td>
| |
| - | <td>• Cut them and run the gel.
| |
| - | </td>
| |
| - |
| |
| - | </tr>
| |
| - | </table></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.10th
| |
| - | Saturday
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="620" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - | <td>• Cut YFP(1A3), RFP(1A3) with S+E
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td>• Purification: RFP(1A3), YFP(1A3)
| |
| - | </td>
| |
| - | <td>• Ligation: RFP+1C3, YFP+1C3
| |
| - | </td>
| |
| - | <td>• Transform the ligation results.</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.11th
| |
| - | Sunday
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="620" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - | <td width="244">• Check the plates.
| |
| - |
| |
| - | </td>
| |
| - | <td width="164">• Culture lig RFY+YFP+IC3<br/>
| |
| - | • Culture 1K3
| |
| - |
| |
| - | </td>
| |
| - | <td>• Colony PCR: RFP(1C3)， YFP(1C3)</td>
| |
| - | </tr>
| |
| - | </table></td>
| |
| - | </tr>
| |
| - |
| |
| - | </table>
| |
| - | <p> </p>
| |
| - | </div>
| |
| - | <div class="block" id="nsheet">
| |
| - | <h3>Week11</h3><hr/>
| |
| - |
| |
| - | <table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1">
| |
| - | <tr><td width="76">Day</td><td width="349">Note</td></tr>
| |
| - |
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.12th
| |
| - | Monday
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table id="intable" width="603" border="0" cellspacing="0" cellpadding="1">
| |
| - |
| |
| - | <td width="191">• Colony PCR: 1A3, 1K3, lig RFP+YFP+1C3.
| |
| - |
| |
| - | </td>
| |
| - |
| |
| - |
| |
| - | <td >• Run the PCR results.
| |
| - |
| |
| - | </td>
| |
| - | <td>• Miniprep: lig RFP+YFP+1C3
| |
| - | </td>
| |
| - |
| |
| - | </table></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.13th
| |
| - | Tuesday
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table id="intable" width="615" border="0" cellspacing="0" cellpadding="1">
| |
| - | <td>• Transform 2M, 2I, 6I, 23L.</td>
| |
| - | <td>• Purification: 1K3</td>
| |
| - |
| |
| - | </table></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.14th
| |
| - | Wednesday
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="640" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - | <td>• Miniprep: 20H, 20J, 22B</td>
| |
| - | <td>• Cut: 1K3 with E+P, lig3 with S+E, 12I(CFP) with P+X,</td>
| |
| - | <td>• Gel excision and /or purification: 1K3, lig3, 12I(CFP)</td>
| |
| - | <td>• Run the gel.<br/>
| |
| - | • Culture: 1I, 1K, 3L, 5E, 7C
| |
| - | </td>
| |
| - | <td>• Miniprep: RFP+YFP+1C3.<br/>
| |
| - | • Run the gel of 1K3, lig3, CFP again.
| |
| - | </td>
| |
| - | <td>• Gel excision and purification<br/>
| |
| - | • Ligation: Lig3+CFP+1K3
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.15th
| |
| - | Thursday
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="618" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - |
| |
| - | <td>• Preserve: 1I, 1K, 3C, 5E, 7C<br/>
| |
| - | • Transform the ligation results
| |
| - | </td>
| |
| - | <td>• Colony PCR: 2M, 2I，6I，23L<br/>
| |
| - | • Cut: 20H, 20J, 22B with X+P
| |
| - |
| |
| - | </td>
| |
| - | <td>• Miniprep: 2M-1, 2I-1, 6I-1, 23L-1<br/>
| |
| - | • Cut: 2M, 2I with E+S
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.16th
| |
| - | Friday
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="627" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - | <td >• Colony PCR: RFP+YFP+1K3</td>
| |
| - | <td>• Cut 13K with E+S, 10I with P+X, 1C3 with E+P
| |
| - | </td>
| |
| - | <td>• Run the gel: 13K, 10I, 1C3, 2I, 2M, 20J, 22H, 22B</td>
| |
| - | <td>• Ligation: 13K+10I+1C3<br/>
| |
| - | • Culture: YFP(Amp), CFP(Amp)
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.17th
| |
| - | Saturday
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="620" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - | <td>• Order primers for 20H, 20J<br/>
| |
| - | • Culture 20H-1, 20J-1, 22B-1
| |
| - | </td>
| |
| - | <td>• Plate the ligation results<br/>
| |
| - | • Culture RFP+YFP+CFP+1K3
| |
| - |
| |
| - | </td>
| |
| - | <td>• Colony PCR: 3A, 1C3<br/>
| |
| - | • Preserve: 1K3, RFP+YFP+1C3.
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.18th
| |
| - | Sunday
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="620" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - | <td width="244">• Miniprep: 20J, 20H, RFP+YFP+CFP+1K3
| |
| - | 1A3
| |
| - | </td>
| |
| - | <td width="164">• Run the gel of PCR results
| |
| - |
| |
| - | </td>
| |
| - | <td>• Cut RFP+YFP+CFP+1K3
| |
| - | with E+P<br/>
| |
| - | • Colony PCR: 13K+10I+1C3
| |
| - | </td>
| |
| - | <td>• PCR: fdhF+rush+Vgb+tetR<br/>
| |
| - | • Preserve: 20H, 20J, 22B
| |
| - | </td>
| |
| - | <td>• Run the gel: 20H, 20J, 22B<br/>
| |
| - | • Cut the bands and purification.
| |
| - | </td>
| |
| - | <td>• Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3<br/>
| |
| - | • Culture: 13K+10I+1C3<br/>
| |
| - | • Grad PCR: fdhF.
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table></td>
| |
| - | </tr>
| |
| - |
| |
| - | </table>
| |
| - | <p> </p>
| |
| - | </div>
| |
| - | <div class="block" id="nsheet">
| |
| - | <h3>Week12</h3><hr/>
| |
| - |
| |
| - | <table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1">
| |
| - | <tr><td width="76">Day</td><td width="349">Note</td></tr>
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| - |
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| - | <tr>
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| - | <td id="sheetleft">Sept.19th
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| - | Monday
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| - |
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| - |
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| - |
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| - | </td>
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| - | <td><table id="intable" width="603" border="0" cellspacing="0" cellpadding="1">
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| - |
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| - | <td width="191">• Miniprep: 13K+10I+1C3, 1C3<br/>
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| - | • Run the gel of digestion results.
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| - |
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| - | </td>
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| - |
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| - |
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| - | <td >• Cut: 13K+10I+1C3, 1A3, fdhF
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| - |
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| - | </td>
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| - | <td>• Run the gel: 20H, 20J, rush+vgb, RYC+1K3, fdhF, 13K+10I, 1A3
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| - | </td>
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| - | <td>• Cut and purification: fdhF, 13K+10I, 1A3<br/>
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| - | • Culture: CFP, YFP
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| - | </td>
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| - | <td>• Purification: Rush PCR<br/>
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| - | • Cut: 22B with X, rush PCR results with S
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| - | </td>
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| - |
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| - | </table></td>
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| - | </tr>
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| - | <tr>
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| - | <td id="sheetleft">Sept.20th
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| - | Tuesday
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| - |
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| - |
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| - |
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| - |
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| - |
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| - |
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| - |
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| - |
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| - | </td>
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| - | <td><table id="intable" width="615" border="0" cellspacing="0" cellpadding="1">
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| - | <td>• Run the 20H, 20J from PCR results.<br/>
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| - | • Transform: fdhF+13K+10I+1A3
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| - | </td>
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| - | <td>• Purification: 20B, Rush<br/>
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| - | • Ligation: 22B+Rush
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| - | </td>
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| - | <td>• Purification: ligation results<br/>
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| - | • Cut: the ligation results with X+S
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| - | </td>
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| - | <td>• PCR 20H<br/>
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| - | • Self-ligation: rush+22M
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| - | </td>
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| - | </table></td>
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| - | </tr>
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| - | <tr>
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| - | <td id="sheetleft">Sept.21st
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| - | Wednesday
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| - |
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| - |
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| - |
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| - |
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| - |
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| - |
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| - |
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| - |
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| - | </td>
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| - | <td><table width="640" border="0" cellspacing="0" cellpadding="1">
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| - | <tr>
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| - | <td>• Colony PCR: fdhF+13K+10I+1A3 on plates</td>
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| - | <td>• Run the gel of PCR results</td>
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| - | <td>• Culture the positive results.</td>
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| - |
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| - | </tr>
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| - | </table>
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| - | </td>
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| - | </tr>
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| - | <tr>
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| - | <td id="sheetleft">Sept.22nd
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| - | Thursday
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| - |
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| - |
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| - |
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| - | </td>
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| - | <td><table width="618" border="0" cellspacing="0" cellpadding="1">
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| - | <tr>
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| - |
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| - | <td>• Miniprep: fdhF+13K+10I+1A3<br/>
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| - | • Preserve the above.
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| - |
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| - | </td>
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| - | <td>• Cut and run the results:
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| - | fdhF+13K+10I+1A3, 13K+10I+1C3
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| - |
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| - |
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| - | </td>
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| - | <td>• Cut: RFP with E+S, YFP with X+P, 1K3 with E+P, 1C3 with E+P
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| - | </td>
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| - | <td>• Purification the digestion results.</td>
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| - | </tr>
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| - | </table>
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| - | </td>
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| - | </tr>
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| - | <tr>
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| - | <td id="sheetleft">Sept.23rd
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| - | Friday
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| - |
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| - |
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| - |
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| - |
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| - |
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| - | </td>
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| - | <td><table width="627" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
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| - | <td >• Ligate: RFP+1A3/ YFP+1C3/1K3, RFP+1A3/1C3</td>
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| - | <td>• Transform the ligation results.
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| - | </td>
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| - |
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| - | </tr>
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| - | </table></td>
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| - | </tr>
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| - | <tr>
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| - | <td id="sheetleft">Sept. 24th
| |
| - | Saturday
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| - |
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| - |
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| - |
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| - |
| |
| - |
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="620" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - | <td>• Order primers for 20H, 20J<br/>
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| - | • Culture 20H-1, 20J-1, 22B-1
| |
| - | </td>
| |
| - | <td>• Plate the ligation results<br/>
| |
| - | • Culture RFP+YFP+CFP+1K3
| |
| - |
| |
| - | </td>
| |
| - | <td>• Colony PCR: 3A, 1C3<br/>
| |
| - | • Preserve: 1K3, RFP+YFP+1C3.
| |
| - |
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td id="sheetleft">Sept.25th
| |
| - | Sunday
| |
| - |
| |
| - | </td>
| |
| - | <td><table width="620" border="0" cellspacing="0" cellpadding="1">
| |
| - | <tr>
| |
| - | <td width="244">• Miniprep: 20J, 20H, RFP+YFP+CFP+1K3
| |
| - | 1A3
| |
| - | </td>
| |
| - | <td width="164">• Run the gel of PCR results
| |
| - |
| |
| - | </td>
| |
| - | <td>• Cut RFP+YFP+CFP+1K3
| |
| - | with E+P<br/>
| |
| - | • Colony PCR: 13K+10I+1C3
| |
| - | </td>
| |
| - | <td>• PCR: fdhF+rush+Vgb+tetR<br/>
| |
| - | • Preserve: 20H, 20J, 22B
| |
| - | </td>
| |
| - | <td>• Run the gel: 20H, 20J, 22B<br/>
| |
| - | • Cut the bands and purification.
| |
| - | </td>
| |
| - | <td>• Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3<br/>
| |
| - | • Culture: 13K+10I+1C3<br/>
| |
| - | • Grad PCR: fdhF.
| |
| - | </td>
| |
| - | </tr>
| |
| - | </table></td>
| |
| - | </tr>
| |
| - |
| |
| - | </table>
| |
| - | <p> </p>
| |
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