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Team:USTC-China/Project/results
From 2011.igem.org
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{{Team:USTC-China/temp/bar4}} | {{Team:USTC-China/temp/bar4}} | ||
| - | ==Verification of ΔcheZ strain <html><a href= "# | + | ==Verification of ΔcheZ strain <html><a href= "https://2011.igem.org/Team:USTC-China/Protocol#Dose-Dependent"><font size="4"><u>(protocol)</u></font></a> </html>== |
<p>The size of colonies of RP1616 was much smaller than that of RP437 under same circumstances and after same period of incubation time. In colony PCR following, RP437 absolutely has much more outcome than RP1616 and we concluded that RP1616 is a ΔcheZ strain.</p> | <p>The size of colonies of RP1616 was much smaller than that of RP437 under same circumstances and after same period of incubation time. In colony PCR following, RP437 absolutely has much more outcome than RP1616 and we concluded that RP1616 is a ΔcheZ strain.</p> | ||
| - | ==Verification of the function of the constructed riboswitch <html><a href= "# | + | ==Verification of the function of the constructed riboswitch <html><a href= "https://2011.igem.org/Team:USTC-China/Protocol#Dose-Dependent"><font size="4"><u>(protocol)</u></font></a> </html>== |
#'''0.25% Semisolid medium'''<br/>[[File:Results11.jpg|center]] | #'''0.25% Semisolid medium'''<br/>[[File:Results11.jpg|center]] | ||
#'''0.4% Semisolid medium'''<br/>[[File:Results11.jpg|center]] | #'''0.4% Semisolid medium'''<br/>[[File:Results11.jpg|center]] | ||
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<p> From the results above we concluded that the constructed riboswitch functions well, especially on 0.3% Semisolid medium.</p> | <p> From the results above we concluded that the constructed riboswitch functions well, especially on 0.3% Semisolid medium.</p> | ||
| - | ==Verification of Toggle-switch <html><a href= "# | + | ==Verification of Toggle-switch <html><a href= "https://2011.igem.org/Team:USTC-China/Protocol#Dose-Dependent"><font size="4"><u>(protocol)</u></font></a> </html>== |
[[File:Results14.jpg]] [[File:Results15.jpg]] | [[File:Results14.jpg]] [[File:Results15.jpg]] | ||
<p>Numbers of colonies with RFP: Numbers of colonies with GFP ≈ 8:25</p> | <p>Numbers of colonies with RFP: Numbers of colonies with GFP ≈ 8:25</p> | ||
| - | ==Modulate Toggle-switches to produce more balanced ratio <html><a href= "# | + | ==Modulate Toggle-switches to produce more balanced ratio <html><a href= "https://2011.igem.org/Team:USTC-China/Protocol#Toggle Switch "><font size="4"><u>(protocol)</u></font></a> </html>== |
[[File:Results6.jpg]] [[File:Results17.jpg]] | [[File:Results6.jpg]] [[File:Results17.jpg]] | ||
<p>Numbers of colonies with RFP: Numbers of colonies with GFP ≈ 6:1</p> | <p>Numbers of colonies with RFP: Numbers of colonies with GFP ≈ 6:1</p> | ||
| - | ==Test of Incorporated Riboswitch into one side of Toggle-switch <html><a href= "# | + | ==Test of Incorporated Riboswitch into one side of Toggle-switch <html><a href= "https://2011.igem.org/Team:USTC-China/Protocol#Toggle Switch"><font size="4"><u>(protocol)</u></font></a> </html>== |
[[File:Results18.jpg]] [[File:Results9.jpg]] | [[File:Results18.jpg]] [[File:Results9.jpg]] | ||
| - | ==Verification of the constructed system’s function <html><a href= "# | + | ==Verification of the constructed system’s function <html><a href= "https://2011.igem.org/Team:USTC-China/Protocol#Toggle Switch-Aptamer-cheZ "><font size="4"><u>(protocol)</u></font></a> </html>== |
[[File:Results110.jpg|center]] | [[File:Results110.jpg|center]] | ||
*6 tubes of constructed monoclonal RP1616 | *6 tubes of constructed monoclonal RP1616 | ||
Revision as of 23:13, 2 October 2011
Verification of ΔcheZ strain (protocol)
The size of colonies of RP1616 was much smaller than that of RP437 under same circumstances and after same period of incubation time. In colony PCR following, RP437 absolutely has much more outcome than RP1616 and we concluded that RP1616 is a ΔcheZ strain.
Verification of the function of the constructed riboswitch (protocol)
- 0.25% Semisolid medium
- 0.4% Semisolid medium
- 0.3% Semisolid medium
From the results above we concluded that the constructed riboswitch functions well, especially on 0.3% Semisolid medium.
Verification of Toggle-switch (protocol)
Numbers of colonies with RFP: Numbers of colonies with GFP ≈ 8:25
Modulate Toggle-switches to produce more balanced ratio (protocol)
Numbers of colonies with RFP: Numbers of colonies with GFP ≈ 6:1
Test of Incorporated Riboswitch into one side of Toggle-switch (protocol)
Verification of the constructed system’s function (protocol)
- 6 tubes of constructed monoclonal RP1616
- Results of the sixth tube
