Team:ZJU-China/Notebook/August

From 2011.igem.org

(Difference between revisions)

Revision as of 04:55, 3 October 2011

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">

Labnote

Biobrick group | Biofilm group

This group...........

July

In this month we......
>>Click to see

August

In this month we......
>>Click to see

September

In this month we......
>>Click to see

Week5

Day

Note

Aug.1st Monday

•Fusion PCR Vgb+YFP+tetR, NirB+RFP+tetR,

•PCR: NirB, Vgb

Aug.2nd Tuesday

•Cut: 13K+10I, 22M+10I

• Purify: 13K+10I, 22M+10I
• Ligation: Pvgb+22M+10I, Pnirb+13K+10I

• PCR backbones
• Electrophresis

• Gel excision and purification

Aug.3rd Wednesday

• Make Phusion Buffer, 5×isothermel buffer

• colony PCR: 20H, 20J, 22B

Aug.4th Thursday

• Cut: 10I+22M, 10I+13; and purification

• Ligation: Pvgb+22M+10I, Pnirb+13K+10I,
• colony PCR: 13K+10I

• Culture: 20H, 20J, 22B, 1K, 1I,3C, 5E, 7C

• Transform: 1G, 3A, 5A, 7A from the distribution plate

Aug.5th Friday

• Check the plates. Contamination, or no positive colonies

• Miniprep: 5 backbones, 22B-1, 22B-3

• PCR: nirB from 13K+10I+nirB to firm the ligation. One positive result.

• Culture the positive colony.

Aug.6th Saturday

Aug.7th Sunday

• Cut: 22M,13K with E & S

• Culture the red colonies from plate of pSB1C3

• PCR: 5 backbones
• Cut the PCR products with P+E and run the gel to confirm.

@@ Line 123: / Line 123: @@
    <div class="bigblock">
 <div class="block" id="nsheet">
-<h3>Week1</h3><hr/>
+<h3>Week5</h3><hr/>
 <table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1">
@@ Line 129: / Line 129: @@
    <tr>
-     <td id="sheetleft">Jul.4th Monday</td>
+     <td id="sheetleft">Aug.1st
-     <td><table id="intable" width="328" border="0" cellspacing="0" cellpadding="1">
+Monday
+</td>
+     <td><table id="intable" width="541" border="0" cellspacing="0" cellpadding="1">
-     <td width="128">• Aerobic cultivation of DH5α</td>
+     <td width="286">•Fusion PCR
+Vgb+YFP+tetR, NirB+RFP+tetR,
+</td>
-     <td width="196">• Preparation of apparatus for the formation of biofilm</td>
+     <td width="251" >•PCR: NirB, Vgb</td>
 </table></td>
    </tr>
    <tr>
-     <td id="sheetleft">Jul 5th Tuesday
+     <td id="sheetleft">Aug.2nd
+Tuesday
 </td>
-     <td>&nbsp;</td>
+     <td><table id="intable" width="615" border="0" cellspacing="0" cellpadding="1">
+      <td width="140"> •Cut: 13K+10I, 22M+10I
+</td>
+    <td width="181" > • Purify: 13K+10I, 22M+10I<br/>
+• Ligation: Pvgb+22M+10I, Pnirb+13K+10I
+</td>
+  <td width="142">• PCR backbones<br/>
+• Electrophresis
+</td>
+  <td width="144">• Gel excision and purification</td>
+</table></td>
    </tr>
    <tr>
-     <td id="sheetleft">Jul.6th
+     <td id="sheetleft">Aug.3rd
 Wednesday
 </td>
-     <td><table width="217" border="0" cellspacing="0" cellpadding="1">
+     <td><table width="640" border="0" cellspacing="0" cellpadding="1">
    <tr>
-     <td width="215">•Receiving  primers ordered previously</td>
+     <td width="170">• Make Phusion Buffer, 5×isothermel buffer
+</td>
+     <td width="184">• colony PCR: 20H, 20J, 22B
+</td>
    </tr>
 </table>
@@ Line 157: / Line 180: @@
    </tr>
    <tr>
-     <td id="sheetleft">Jul.7th
+     <td id="sheetleft">Aug.4th
 Thursday
 </td>
-     <td><table width="238" border="0" cellspacing="0" cellpadding="1">
+     <td><table width="618" border="0" cellspacing="0" cellpadding="1">
    <tr>
-     <td width="200">• Preparation of the aliquot of the primers</td>
+     <td >• Cut: 10I+22M, 10I+13; and purification
-    <td width="200">• Something wrong with a shaking incubator</td>
+</td>
+<td>• Ligation: Pvgb+22M+10I, Pnirb+13K+10I,<br/>
+• colony PCR: 13K+10I
+</td>
+<td>• Culture: 20H, 20J, 22B, 1K, 1I,3C, 5E, 7C</td>
+<td>• Transform: 1G, 3A, 5A, 7A from the distribution plate</td>
    </tr>
 </table>
@@ Line 169: / Line 200: @@
    </tr>
    <tr>
-     <td id="sheetleft">Jul.8th
+     <td id="sheetleft">Aug.5th
 Friday
 </td>
-     <td><table width="222" border="0" cellspacing="0" cellpadding="1">
+     <td><table width="627" border="0" cellspacing="0" cellpadding="1">
        <tr>
-        <td width="155">• Preparation of culture plates for the transformations</td>
+       <td >• Check the plates. Contamination, or no positive colonies</td>
+       <td>• Miniprep: 5 backbones, 22B-1, 22B-3</td>
+       <td>• PCR: nirB from 13K+10I+nirB to firm the ligation. One positive result.</td>
+       <td>• Culture the positive colony.</td>
        </tr>
      </table></td>
    </tr>
    <tr>
-     <td id="sheetleft">Jul.9th
+     <td id="sheetleft">Aug.6th
 Saturday
 </td>
-     <td><table width="313" border="0" cellspacing="0" cellpadding="1">
+     <td><table width="620" border="0" cellspacing="0" cellpadding="1">
    <tr>
-     <td width="127">• Preparation of culture plates for the transformations</td>
-    <td width="182">• protocols of transformation</td>
    </tr>
 </table>
@@ Line 191: / Line 227: @@
    </tr>
    <tr>
-     <td id="sheetleft">Jul.10th
+     <td id="sheetleft">Aug.7th
 Sunday
 </td>
-     <td><table width="246" border="0" cellspacing="0" cellpadding="1">
+     <td><table width="620" border="0" cellspacing="0" cellpadding="1">
        <tr>
-         <td>• Several colonies were picked up and cultivated in  5mL LB medium.
+         <td width="244">• Cut: 22M,13K with E &amp; S
-•Cryosectioning of biofilm
+</td>
+<td width="164">• Culture the red colonies from plate of pSB1C3</td>
+<td>• PCR: 5 backbones<br/>
+• Cut the PCR products with P+E and run the gel to confirm.
 </td>
        </tr>
@@ Line 204: / Line 244: @@
 </table>
-<p>&nbsp;</p>
+ <p>&nbsp;</p>
 </div>