Team:Peking S/lab/protocol

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<font size=6> <font color="#FFFFFF">Protocol</font></FONT>
 
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==Peking S 2011==
 
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/DDDP"><font size=3><font color=#FFFFFF>*DNA Double Digestion Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/8/84/DNA_double_digestion_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
 
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/DGEP"><font size=3><font color=#FFFFFF>*DNA Gel Extraction Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/8/83/DNA_Gel_extraction_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
 
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/MP"><font size=3><font color=#FFFFFF>*Miniprep Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/1/19/Miniprep_Protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
 
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PCIEG"><font size=3><font color=#FFFFFF>*Protocol for Chemical Inducible Expression of GFP</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/0/06/Protocol_for_chemical_inducible_expression_of_GFP.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
 
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PDPRM"><font size=3><font color=#FFFFFF>*Protocol for DNA Purification from Reaction Mixture</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/b/bf/Protocol_for_DNA_purification_from_reaction_mixture.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
 
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PLIDPVD"><font size=3><font color=#FFFFFF>*Protocol for Ligation of Insert DNA into Plasmid Vector DNA</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/0/04/Protocol_for_ligation_of_insert_DNA_into.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
 
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<font size=3><font color=#FFFFFF>*Protocol for PCR with EasyPfu DNA Polymerase Preparation for Reaction</font></font>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/d/da/Protocol_for_PCR_with_EasyPfu_DNA_Polymerase_Preparation_for_Reaction_Mixture.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
 
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PPCCT"><font size=3><font color=#FFFFFF>*Protocol for Preparation of Competent Cells for Transformation</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/4/43/Protocol_for_preparation_of_competent_cells_for_transformation.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
 
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/SDMP"><font size=3><font color=#FFFFFF>*Site-directed Mutagenesis Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/d/d0/Site_directed_mutagenesis_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
 
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/TP"><font size=3><font color=#FFFFFF>*Transformation Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/7/75/Transformation_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
 
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Revision as of 08:27, 2 October 2011


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Protocol


Double Digestion |Gel Extraction|Miniprep | Chemical Inducible Expression of GFP |DNA Purification |Ligation of Insert DNA | PCR |Preparation of Competent Cells| Site-directed Mutagenesis| Transformation|


           *SDS-PAGE & western blot protocol         
             download PDF version

           *SDS-PAGE & western sample preparation protocol         
             download PDF version

           *Protocol for chemical inducible expression of MBP & sample preparation         
             download PDF version

           *DNA Double Digestion Protocol         
             download PDF version

           *DNA Gel Extraction Protocol         
             download PDF version

           *Miniprep Protocol         
             download PDF version

           *Protocol for Chemical Inducible Expression of GFP         
             download PDF version

           *Protocol for DNA Purification from Reaction Mixture         
             download PDF version

           *Protocol for Ligation of Insert DNA into Plasmid Vector DNA         
             download PDF version

           *Protocol for PCR with EasyPfu DNA Polymerase Preparation for Reaction         
             download PDF version

           *Protocol for Preparation of Competent Cells for Transformation         
             download PDF version

           *Site-directed Mutagenesis Protocol         
             download PDF version

           *Transformation Protocol         
             download PDF version