Team:Peking S/lab/protocol

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Protocol

Peking S 2011

__NOTOC__ {{Template:Http://2011.igem.org/Team:Peking_S/box4extra}} {{Template:Http://2011.igem.org/Team:Peking S/background}} Protocol {{Template:Http://2011.igem.org/Team:Peking S/protocal}}
*SDS-PAGE & western blot protocol

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*SDS-PAGE & western sample preparation protocol

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*Protocol for chemical inducible expression of MBP & sample preparation

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*DNA Double Digestion Protocol

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*DNA Gel Extraction Protocol

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*Miniprep Protocol

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*Protocol for Chemical Inducible Expression of GFP

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*Protocol for DNA Purification from Reaction Mixture

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*Protocol for Ligation of Insert DNA into Plasmid Vector DNA

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*Protocol for PCR with EasyPfu DNA Polymerase Preparation for Reaction

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*Protocol for Preparation of Competent Cells for Transformation

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*Site-directed Mutagenesis Protocol

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*Transformation Protocol

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+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/DDDP"><font size=3><font color=#FFFFFF>*DNA Double Digestion Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/8/84/DNA_double_digestion_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/DGEP"><font size=3><font color=#FFFFFF>*DNA Gel Extraction Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/8/83/DNA_Gel_extraction_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/MP"><font size=3><font color=#FFFFFF>*Miniprep Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/1/19/Miniprep_Protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PCIEG"><font size=3><font color=#FFFFFF>*Protocol for Chemical Inducible Expression of GFP</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/0/06/Protocol_for_chemical_inducible_expression_of_GFP.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PDPRM"><font size=3><font color=#FFFFFF>*Protocol for DNA Purification from Reaction Mixture</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/b/bf/Protocol_for_DNA_purification_from_reaction_mixture.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PLIDPVD"><font size=3><font color=#FFFFFF>*Protocol for Ligation of Insert DNA into Plasmid Vector DNA</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/0/04/Protocol_for_ligation_of_insert_DNA_into.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<font size=3><font color=#FFFFFF>*Protocol for PCR with EasyPfu DNA Polymerase Preparation for Reaction</font></font>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/d/da/Protocol_for_PCR_with_EasyPfu_DNA_Polymerase_Preparation_for_Reaction_Mixture.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PPCCT"><font size=3><font color=#FFFFFF>*Protocol for Preparation of Competent Cells for Transformation</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/4/43/Protocol_for_preparation_of_competent_cells_for_transformation.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/SDMP"><font size=3><font color=#FFFFFF>*Site-directed Mutagenesis Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/d/d0/Site_directed_mutagenesis_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/TP"><font size=3><font color=#FFFFFF>*Transformation Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/7/75/Transformation_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br><br>
+</div>
+<div id="notebook home">
+<div id="titlegreen">
+<br><br>
+<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</div>
+__NOTOC__
+{{Template:Http://2011.igem.org/Team:Peking_S/box4extra}}
+{{Template:Http://2011.igem.org/Team:Peking S/background}}
+<font size=6> <font color="#FFFFFF">Protocol</font></FONT>
+{{Template:Http://2011.igem.org/Team:Peking S/protocal}}
+<html>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/SDS&WB"><font size=3><font color=#FFFFFF>*SDS-PAGE & western blot protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/7/7e/SDS-PAGE_%26_western_blot_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/SDS&W"><font size=3><font color=#FFFFFF>*SDS-PAGE & western sample preparation protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/f/f8/SDS-PAGE_%26_western_sample_preparation_protocol-xteng.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/MBP"><font size=3><font color=#FFFFFF>*Protocol for chemical inducible expression of MBP & sample preparation</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/0/08/Protocol_for_chemical_inducible_expression_of_MBP_%26_sample_preparation.pdf" target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
 <br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;