Team:Peking S/lab/notebook/dln

Linna Deng's Notebook

summary

After designed the gene circuit in cell A, I constructed a duoble-plasmids stucture containing the expression of signal molecules and death protein. One of the plasmid is consisted of an PBad promoter without AraC, the gene expressing mCherry, and lasI protein generator. the other is made up with promoter for salicylic acid and ccdB gene which is a control of cell death. I used PCR to confirm whether the plasmids containing the genes were in correct size.

Contents

• July, 2011

• August, 2011

• September, 2011

• October, 2011

July

Mon	Tue	Wed	Thu	Fri	Sat	Sun
-	-	-	-	1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30	31
-	-	-	-	-	-	-

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7.3-7.9

Designed the structure of cell A, which was consisted of two plasmids:

PBad+RBS+RFP-tag+RBS+lasI+terminator

PSal+RBS+ccdB+terminator

7.10

7.12

7.13

7.14

7.15

7.16

7.18

7.19

7.20

7.21

7.22

7.23

7.29

7.30

7.31

August

Mon	Tue	Wed	Thu	Fri	Sat	Sun
1	2	3	4	5	6	7
8	9	10	11	12	13	14
15	16	17	18	19	20	21
22	23	24	25	26	27	28
29	30	31	-	-	-	-

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8.1

8.2

8.4

8.5

8.6

8.7

8.8

8.9

8.10

8.11

8.12

8.13

8.14

8.15

8.16

8.17

8.18

8.19

8.20

8.21

8.22

8.23

8.25

8.26

8.27

8.30

8.31

September

Mon	Tue	Wed	Thu	Fri	Sat	Sun
	-	-	1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	-	--

[TOP]

9.1

9.2

9.3

9.6

9.7

9.8

9.9

9.12

9.13

9.14

9.15

9.16

9.17

9.21

9.22

9.23

9.24

9.25

9.26

9.27

9.28

9.29

9.30

October

Mon	Tue	Wed	Thu	Fri	Sat	Sun
-	-	-	-	1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	-	-	-	-	-	-

[TOP]

10.1

10.3

10.4

10.5

10.7

10.8

10.9

10.10

10.11

10.12

10.13

10.15

10.16-10.21

10.21-10.25