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Team:Peking S/lab/notebook
From 2011.igem.org
(→Wenhan Luo) |
(→Yanrong Ma) |
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| - | + | 6.24-7.5 | |
| + | Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion. | ||
| + | 7.6-7.7 | ||
| + | Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing. | ||
| + | 7.8-7.12 | ||
| + | Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome. | ||
| + | 7.13-7.23 | ||
| + | Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters. | ||
| + | 7.24-8.13 | ||
| + | Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules. | ||
| + | 8.14-9.5 | ||
| + | Testing A-factor system performance. | ||
| + | 9.6-9.18 | ||
| + | Construction cinI/cinR system mainly contains constitutively expressed cinR and a gfp controlled by cinR and cinI synthesized chemical molecules. | ||
| + | 9.19-10.1 | ||
| + | Characterization of cinI/cinR system. | ||
| + | |||
[https://2011.igem.org/Team:Peking_S/lab/notebook/myr go to his homepage] | [https://2011.igem.org/Team:Peking_S/lab/notebook/myr go to his homepage] | ||
| + | |||
== <font color="#ffffff"> <font size=4>Hanchi Lin</font></font> == | == <font color="#ffffff"> <font size=4>Hanchi Lin</font></font> == | ||
Revision as of 04:45, 1 October 2011
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Attribution
Here is a brief introduction of each member's task.If you're looking for detailed information,look into the member's personal note.
Handuo Shi
my task is...blah blah
Dingqiao Wen
my task is...blah blah
Yiwei Chen
my task is...blah blah
Yanrong Ma
6.24-7.5
Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion.
7.6-7.7
Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing.
7.8-7.12
Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome.
7.13-7.23
Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters.
7.24-8.13
Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules.
8.14-9.5
Testing A-factor system performance.
9.6-9.18
Construction cinI/cinR system mainly contains constitutively expressed cinR and a gfp controlled by cinR and cinI synthesized chemical molecules.
9.19-10.1
Characterization of cinI/cinR system.
Hanchi Lin
my task is...blah blah
== Wenhan Luo
my task is...blah blah
Jingchen Feng
my task is...blah blah
Jingyi Xi
Mainly in charge of microfluidic device, I create a two-layered high-throughput microfluidic system for our project, which provides a suitable space for different cells to be separated apart and population controlled, as well as allowing chemical wires to transfer freely from each side to the other. What’s more, I also take part in molecular cloning of TPP regulator, characterization of chemical wire toolbox, and modeling work of XOR gate.
Xiao Qin
my task is...blah blah
Rui Sun
my task is...blah blah
Ruofan Yu
Since I'm mainly in charge of the team wiki and other chores,I don't have a specific task in our project.But I do help other teammates when they're having too many things on their to-do list.Please refer to Handuo Shi and Weiyue Ji's lab diary to get detailed imformation.
Linna Deng
my task is...blah blah
Weiyue Ji
my task is...blah blah
