Team:UTP-Panama/Experiments
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According to the characterization made by Team Edinburgh 2009, the Pyear promoter (BBa_K216005) used by Bristol 2010, works best in minimal media and complex media with a nitrate concentration of 40mM. Using this reference, we added 40mM of KNO3 to every growth media in the different temperatures for Bristol 2010 - BBa_K381001 and UTP-Panama 2011 – BBa_K672000.<br> | According to the characterization made by Team Edinburgh 2009, the Pyear promoter (BBa_K216005) used by Bristol 2010, works best in minimal media and complex media with a nitrate concentration of 40mM. Using this reference, we added 40mM of KNO3 to every growth media in the different temperatures for Bristol 2010 - BBa_K381001 and UTP-Panama 2011 – BBa_K672000.<br> | ||
- | 5. We used nine falcon tubes of | + | 5. We used nine falcon tubes of 15 mL. Three tubes for every different substrate at the three temperatures.<br> |
- | 6. To each tube we added 10 ml of liquid LB, | + | 6. To each tube we added 10 ml of liquid LB, Minimal Media or Saline Solution and KNO3 40 mM.<br> |
7. Tubes corresponding the different substrates were inoculated with BBa_K381001.<br> | 7. Tubes corresponding the different substrates were inoculated with BBa_K381001.<br> | ||
- | 8. Tubes were incubated at | + | 8. Tubes were incubated at 37°C, 8°C and 23°C for 24 hours.<br> |
Revision as of 04:20, 29 September 2011
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Experiment 1 | Experiment 2 | Experiments 3 | BCCS-Bristol 2010 BiobrickFirst Experience with BBa_k381001Objective:
1. We used six falcon tubes of 25 mL. Three tubes were used for inoculation of the colonies from 06/09/11 and 12/09/11. 2. Two positive controls were prepared and one negative control for each test. 3. To positive control tubes we added 10 ml of liquid LB, 9.7 uL of chloramphenicol (33μg/mL) and 5.00 µL of KNO3 50 mM. 4. To negative control tube we added the same amount of LB and Chloramphenicol specified in the previous line, except KNO3.
5. Each tube was inoculated with the colonies; three of them with the conlonies from 06/09/11 and the rest with colonies from 12/09/11. 6. Tubes were incubated at 37 ° C for 48 hours.
Second ExperienceObjectives:
5. We used nine falcon tubes of 15 mL. Three tubes for every different substrate at the three temperatures. |