Team:Queens Canada/Side/Biodegradation

Upon discovering that a bacterium, Pseudomonas putida, has the ability to degrade naphthalene through a specialized pathway, we worked on a proposed construct that serves as a thought experiment on the ability of C. elegans to acquire biodegradation capability. The P. putida pathway is extensive, however, we propose that with enough time and resources, the entire pathway could be engineered in C. elegans to allow the worm to eat up naphthalene.

In this project, we decided to focus on the biodegradation of Naphthalene. Naphthalene is an environmental contaminant that can be formed in industrial processes. Naphthalene is a polyaromatic hydrocarbon (PAH) and is considered to be a potential carcinogen(1) . This chemical is a good target for bioremediation and can be broken down by the NAH7 operon of the P. putida. bacteria.