• Requires phycocyanobilin biosynthetic genes (BBa_I15008 and BBa_I15009) for chromophore formation.
• Must be used in E. coli deficient in natural EnvZ.

Expecting function (without experimental proof): Exposure to green light (535 nm) phosphorylate EnvZ histidin kinase domain. The activated EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpR positive promoter (BBa_R0083). Ampicillin resistant.

Output: PoPS Usage and Biology: pBAD is an E.coli promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription. K206000 is a variant pBAD promoter with a modified AraI1 site that has been shown both to be responsive to lower concentrations of arabinose and to exhibit a higher maximum expression than the wild type (BBa_I13453), as measured by coupling to a fluorescent reporter. What you can do with it: At a given level of arabinose input, BBa_K206000 will provide a higher level of PoPS output than its family members, allowing analog device responses. See our wikifor a project that makes use of this property. Compatibility: Chassis: Best used in the E. coli strain BW27783, which has been modified to permit homogeneous pBAD promoter expression by substituting the chromosomal arabinose-dependent promoter of AraE (arabinose transporter protein) with a constitutive promoter. Backbone: Has been shown to work on plasmid pSB1C3. Reporter: Has been shown to work with reporters BBa_I13507 and BBa_I763020. Ampicillin resistant.

Output: PoPS Usage and Biology: pBAD is an E.coli promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription. K206001 is a variant pBAD promoter with a modified AraI1 site that has been shown to be less responsive at low concentrations of arabinose and to exhibit a lower maximum expression than the wild type (BBa_I13453), as measured by coupling to a fluorescent reporter. What you can do with it: At a given level of arabinose input, BBa_K206001 will provide a lower level of PoPS output than its family members, allowing analog device responses. See our wikifor a project that makes use of this property. Compatibility: Chassis: Best used in the E. coli strain BW27783, which has been modified to permit homogeneous pBAD promoter expression by substituting the chromosomal arabinose-dependent promoter of AraE (arabinose transporter protein) with a constitutive promoter . Backbone: Has been shown to work on plasmid pSB1C3. Reporter: Has been shown to work with reporters BBa_I13507 and BBa_I763020. Ampicillin resistant.