Team:Panama/16 June 2011

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(Created page with " We autoclaving 1.5ml eppendorff tubes and then were treat at 800C for 2h in the microwave for nuclease elimination.  Orlando prepare chloranfenicol (100µg/ml)  We pass...")
 
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 We autoclaving 1.5ml eppendorff tubes and then were treat at 800C for 2h in the microwave for nuclease elimination.
 We autoclaving 1.5ml eppendorff tubes and then were treat at 800C for 2h in the microwave for nuclease elimination.
-
 Orlando prepare chloranfenicol (100µg/ml)
+
 
-
 We pass our Biobrick from Agar medium +chloranfenicol to LB medium + Chloranfenicol.
+
 Orlando prepare chloramphenicol (100µg/ml)
-
 Sandra use as negative control : 200µl plasmid (RBS+T) in medium with Amp and inoculate in LB medium with Chloranfenicol. No colonies have to grow because not have resistance for chloranfenicol
+
 
 +
 We pass our Biobrick from Agar medium + chloramphenicol to LB medium + Chloramphenicol.
 +
 
 +
 Sandra use as negative control : 200µl plasmid (RBS+T) in medium with Amp and inoculate in LB medium with Chlorampheenicol. No colonies have to grow because not have resistance for chloramphenicol.
 +
 
 Incubate at 370 C in shaker.
 Incubate at 370 C in shaker.

Latest revision as of 04:27, 29 September 2011

 We autoclaving 1.5ml eppendorff tubes and then were treat at 800C for 2h in the microwave for nuclease elimination.

 Orlando prepare chloramphenicol (100µg/ml)

 We pass our Biobrick from Agar medium + chloramphenicol to LB medium + Chloramphenicol.

 Sandra use as negative control : 200µl plasmid (RBS+T) in medium with Amp and inoculate in LB medium with Chlorampheenicol. No colonies have to grow because not have resistance for chloramphenicol.

 Incubate at 370 C in shaker.

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