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Team:Panama/13 July 2011
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Apparently EcoR1 enzyme cut the plasmid, we see bands of approximately 2 kb and others of 1,2 kb. We think that the gen isn't in the plasmid because the weight desired es of 4,4 kb (4,441pb) | Apparently EcoR1 enzyme cut the plasmid, we see bands of approximately 2 kb and others of 1,2 kb. We think that the gen isn't in the plasmid because the weight desired es of 4,4 kb (4,441pb) | ||
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| + | We have to do electrophoresis again for: | ||
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| + | -Check if primers weren't degraded, Results: There aren't | ||
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| + | -If gen is mutated: | ||
| + | Good news: Sample 6 of 10/30/11- mutated gen! | ||
| + | |||
| + | Bad news: rh1AB + psB1C3 of 10/30/11 isn't observed. | ||
Revision as of 02:37, 29 September 2011
July 13, 2011
We did:
1. Electrophoresis of the products of yesterday PCR and 3 digestions.
What we expect to know? If EcoR1 enzyme digest and in how much microliters is efficient, that with dilution the amplification be more effective.
Results: We had not results of PCR :( !
Apparently EcoR1 enzyme cut the plasmid, we see bands of approximately 2 kb and others of 1,2 kb. We think that the gen isn't in the plasmid because the weight desired es of 4,4 kb (4,441pb)
We have to do electrophoresis again for:
-Check if primers weren't degraded, Results: There aren't
-If gen is mutated: Good news: Sample 6 of 10/30/11- mutated gen!
Bad news: rh1AB + psB1C3 of 10/30/11 isn't observed.
