Team:ITESM Mexico/Assembly
From 2011.igem.org
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- | [[File: | + | [[File:Const.Promoter.jpg|100px|caption]] |
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- | ''' | + | '''BBa_J23109:''' Constitutive promoter family. |
+ | Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. All parts except J23119 are present in plasmid J61002. Part J23119 is present in pSB1A2. This places the RFP downstream of the promoter. Reported activities of the promoters are given as the relative fluorescence of these plasmids in strain TG1 grown in LB media to saturation. See part J61002 for details on their use. These promoter parts can be used to tune the expression level of constitutively expressed parts. The NheI and AvrII restriction sites present within these promoter parts make them a scaffold for further modification. | ||
+ | Ampicillin resistant. | ||
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+ | [[File:RBS.jpg|100px|caption]] | ||
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+ | '''BBa_B0034:''' RBS based on Elowitz repressilator. | ||
+ | Ampicillin resistant. | ||
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+ | [[File:HO1.jpg|100px|caption]] | ||
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+ | '''BBa_I15008:''' One of two requisite genes required for the biosynthesis of phycocyanobilin from heme. | ||
+ | Usage and Biology | ||
+ | Ho1 oxidizes the heme group using a ferredoxin cofactor, generating biliverdin IXalpha and representing the first of two steps in phycocyanobilin (PCB) biosynthesis. PCB associates with Cph8, creating a light responsive protein complex. Functions in tandem with BBa_I15009 for PCB biosynthesis. PCB then associates withPart:BBa_I15010, a light responsive Cph8/EnvZ fusion protein. | ||
+ | Kanamycin resistant. | ||
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+ | [[File:pcyA.jpg|100px|caption]] | ||
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+ | '''BBa_I15009:''' Phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis | ||
+ | Second of two required phycocyanobilin biosynthetic genes. | ||
+ | Usage and Biology: Converts biliverdin IXalpha (BV)(produced by Part:BBa_I15008) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes. Associates with Part:BBa_I15010 to transduce a light signal into a genetic response. Kanamycin resistant. | ||
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+ | [[File:CFP.jpg|100px|caption]] | ||
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+ | '''BBa_E0022:''' Enhanced cyan fluorescent protein derived from A. victoria GFP | ||
+ | Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. | ||
+ | Ampicillin resistant. | ||
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Revision as of 01:43, 29 September 2011