Team:Queens Canada/Project/Methods
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<regulartext> Since <i>C. elegans</i> is a multicellular organism, we needed to make sure our non-native GPCR was expressed in the neurons that would stimulate our worm to move towards the ligand. To accomplish this, we researched GPCRs that would normally be expressed in the olfactory (“smelling”) neurons of the worm. Then, we kept the promoter of the native GPCR and substituted the rest of the protein with our foreign GPCR. Hence, our constructs consist of a <i>C. elegans</i> promoter, followed by a non-native GPCR and terminated by the <i>C. elegans</i> <span class="classredt"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K309012">UNC-54 terminator</a><span> that our team researched last year.</regulartext><p> | <regulartext> Since <i>C. elegans</i> is a multicellular organism, we needed to make sure our non-native GPCR was expressed in the neurons that would stimulate our worm to move towards the ligand. To accomplish this, we researched GPCRs that would normally be expressed in the olfactory (“smelling”) neurons of the worm. Then, we kept the promoter of the native GPCR and substituted the rest of the protein with our foreign GPCR. Hence, our constructs consist of a <i>C. elegans</i> promoter, followed by a non-native GPCR and terminated by the <i>C. elegans</i> <span class="classredt"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K309012">UNC-54 terminator</a><span> that our team researched last year.</regulartext><p> | ||
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Revision as of 00:51, 29 September 2011
Mus musculus-NPY1R_Mus
Rattus novergicus-MARM1
Rattus novergicus-MARM
Homo Sapiens-OX1R
2. Kiyohara H, Torigoe S, Kaida N, Asaki T, Iida, T, Hayashi H and Takizawa N. (1994) Cloning and Characterization of a Chromosomal Gene Cluster, pah, That Encodes the Upper Pathway for Phenanthrene and Naphthalene Utilization by Pseudomonas putida OUS82. Journal of Bacteriology, 176:2439-2443.
3. Ahmed S and Hodgkin J. (2000) MRT-2 checkpoint protein is required for germline immortality and telomere replication in C. elegans. Nature, 403(6766):149-151.