Team:UANL Mty-Mexico/Contributions/Parts

     <td class="final"><b>OL region from Lambda phage</b> allows biphasic switch <a href="http://partsregistry.org/Part:BBa_K566002" target="_new" >(K566002)</a> effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter <a href="http://partsregistry.org/Part:BBa_K566001" target="_new" >(K566001)</a>.</td>

     <td class="final"><b>pRM promoter from Lambda</b> phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] induce it, high [cI] repress it. It needs OL region <a href="http://partsregistry.org/Part:BBa_K566000" target="_new" >(K566000)</a> for effective repression. Makes the biphasic switch <a href="http://partsregistry.org/Part:BBa_K566002" >(K566002)</a> together with OL region. </td>

     <td class="final">The <b>Biphasic Switch</b> combines positive and negative regulation through a single input. It is turned ON by low lambda cI concentrations and OFF by high cI concentrations.</td>

     <td class="final"> <b>CFP optimized for <i>E. coli</i>.</b> Cyan fluorescent protein optimized from part <a href="http://partsregistry.org/Part:BBa_E0022" target="_new">BBa_E0022</a> with preferential codon usage for improved expression in <i>E. coli</i>. It is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea victoria . It possesses bright fluorescence with exitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1.</td>

     <td class="final"><b>Protein generator of cyan fluorescent protein without transcription terminator.</b> It may be negatively regulated by Mnt repressor <a href="http://partsregistry.org/Part:BBa_C0072" target="_new">(BBa_C0072)</a>.</td>

     <td class="final"><b>pCpcG2 promoter (Green light inducible).</b> Green light inducible pCpcG2 promoter from pJT122 plasmid constructed by Tabor et al. (2010). It is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light. Light intensities must be carefully regulated to achieve successful gene expression.</td>

     <td class="final"> <b>penI-pCpcG2-pPenI-mnt.</b> Repression-based potential AND gate. Mnt Repressor is under the control of the PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter. pCpcG2 promoter is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light.</td>

     <td class="final"><b>PenI repressor optimized for E. coli (inverted sequence).</b> Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in <i>E. coli</i>. PenI negatively regulates transcription from pPenI promoter <a href="http://partsregistry.org/Part:BBa_R0074" target="_new">(BBa_R0074)</a>.</td>

     <td class="final"><b>Mnt repressor optimized for <i>E. coli</i>.<b> Mnt repressor optimized from part BBa_C0072 with preferential codon usage for improved expression in <i>E. coli</i>. It acts on pMnt promoter <a href="http://partsregistry.org/Part:BBa_R0073" target="_new">(BBa_R0073)</a>.</td>

     <td class="final"><b>pCpcG2 promoter, inverted sequence (Green light inducible).</b> Inverted Green light inducible pCpcG2 promoter, from pJT122 plasmid constructed by Tabor et al. (2010). It is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light. Light intensities must be carefully regulated to achieve successful gene expression. The sequence was inverted to ease DNA synthesis.</td>

     <td class="final"><b>PenI repressor optimized for E. coli (inverted sequence).</b> Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in <i>E. coli</i>. Negatively regulates transcription from pPenI promoter. <a href="http://partsregistry.org/Part:BBa_R0074" target="_new">(BBa_R0074)</a>. The nucleotide sequence was inverted to ease DNA synthesis.</td>

     <td class="final"><b>cI434 repressor from phage 434 optimized for E. coli cI (+LVA).</b> Optimized cI434 repressor with LVA tag from phage 434, with preferential codon usage for improved expression in <i>E. coli</i>.</td>

     <td class="final"><b>cI repressor from Lambda phage optimized for <i>E. coli</i>.</b> Includes LVA tag for faster degradation.<br/>

     <td class="final"><b>RFP optimized for <i>E. coli</i> (+LVA).</b> Includes LVA tag for faster degradation.<br/>

     <td class="final"><b>pRM-RBS-cI434.</b> Composite part for Biphasic switch tests. Include pRM promoter from phage λ pRM promoter from Lambda phage. It may be positively and negatively regulated according to cI protein concentration. Low [cI] turn it ON, high [cI] turn it OFF. It contains three operators named OR1, OR2 and OR3, all cI binding sites. When bound to OR1 and OR2, cI positively regulates pRM; cI binding to OR3 repress pRM. However, due to a low binding affinity to OR3, higher concentrations of the protein are required. <br/>

     <td class="final"> <b>OL-pTetMnt-cI.</b> Induction system for Biphasic Switch. <br/>

     <td class="final"><b>pRM434-RFP.</b> Red Fluorescent Protein under the control of pRM434 promoter. It was constructed for demonstrating dual state of a biphasic switch. </td>

     <td class="final"><b>E. coli MxRed.</b> Derived from Dr. Jeff Tabor JT2 <i>E. coli</i> strain, this part is a built-in red-light induction system in <i>E. coli</i> made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher. <br/>

     <td class="final"><b>Mnt repressor regulated by pOmpC.</b> </td>

     <td class="final"><b>pcyA CDS.</b></td>

     <td class="final"><b>ho1 CDS</b></td>

     <td class="final"><b>pcyA gene (constitutive expression).</b> pcyA gene transcribed from constitutive promoter.</td>

     <td class="final"><b>ho1 gene (constitutive expression).</b> ho1 gene transcribed from constitutive promoter. ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB). </td>

     <td class="final"><b>Cph8 CDS.</b> The red light-sensing protein Cph8 is expressed in the phosphorylated ground state. It is switched to the unphosphorylated state by 650-nm light and back to the phosphorylated state by 705-nm light. When phosphorylated, Cph8 passes a phosphoryl group to OmpR, which then binds to and activates transcription from PompC. Because it is inactivated by red light, Cph8 can be considered a logical (NOT red) sensor. A genetic inverter or logical NOT gate is used to invert the response of the (NOT red) sensor to that of a red light sensor.</td>

     <td class="final"><b>Cph8 gene for red-photoreceptor (constitutive expression).</b> Cassette for constitutive expression of Cph8, originally constructed by Dr. Jeff Tabor.<br/>

     <td class="final"><b>ho1 CDS</b></td>

     <td class="final"><b>ho1 CDS</b></td>

     <td class="final"><b>ho1 CDS</b></td>

     <td class="final"><b>ho1 CDS</b></td>

     <td class="final"><b>ho1 CDS</b></td>