Team:UNAM-Genomics Mexico/Project

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Among the biological systems that produce hydrogen, the most efficient ones achieve it through reactions catalyzed by enzymes with iron-sulfur clusters which require hypoxic microenvironments to work. The bacterium ''Rhizobium etli'', during its symbiotic relationship with the common bean ''Phaseolus vulgaris'', can transform nitrogen gas into ammonia in a process called nitrogen fixation. In exchange the plant provides the bacteria with carbon sources and a protected niche inside its root, where ''Rhizobium etli'' reaches a hypoxic state. We will exploit this microenvironment to produce hydrogen in ''Rhizobium etli'' introducing a pathway assembled with elements from ''Clostridium acetobutylicum'', ''Desulfovibrio africanus'' and ''Chlamydomonas reinhardtii'', while maintaining nitrogen fixation. The two goals of our project are to make ''Rhizobium etli'' a powerful agent in environmental protection by nitrifying soils and producing hydrogen from solar energy, and to standardize the work in Rhizobials.
Among the biological systems that produce hydrogen, the most efficient ones achieve it through reactions catalyzed by enzymes with iron-sulfur clusters which require hypoxic microenvironments to work. The bacterium ''Rhizobium etli'', during its symbiotic relationship with the common bean ''Phaseolus vulgaris'', can transform nitrogen gas into ammonia in a process called nitrogen fixation. In exchange the plant provides the bacteria with carbon sources and a protected niche inside its root, where ''Rhizobium etli'' reaches a hypoxic state. We will exploit this microenvironment to produce hydrogen in ''Rhizobium etli'' introducing a pathway assembled with elements from ''Clostridium acetobutylicum'', ''Desulfovibrio africanus'' and ''Chlamydomonas reinhardtii'', while maintaining nitrogen fixation. The two goals of our project are to make ''Rhizobium etli'' a powerful agent in environmental protection by nitrifying soils and producing hydrogen from solar energy, and to standardize the work in Rhizobials.

Revision as of 23:57, 28 September 2011

UNAM-Genomics_Mexico


Project

Among the biological systems that produce hydrogen, the most efficient ones achieve it through reactions catalyzed by enzymes with iron-sulfur clusters which require hypoxic microenvironments to work. The bacterium Rhizobium etli, during its symbiotic relationship with the common bean Phaseolus vulgaris, can transform nitrogen gas into ammonia in a process called nitrogen fixation. In exchange the plant provides the bacteria with carbon sources and a protected niche inside its root, where Rhizobium etli reaches a hypoxic state. We will exploit this microenvironment to produce hydrogen in Rhizobium etli introducing a pathway assembled with elements from Clostridium acetobutylicum, Desulfovibrio africanus and Chlamydomonas reinhardtii, while maintaining nitrogen fixation. The two goals of our project are to make Rhizobium etli a powerful agent in environmental protection by nitrifying soils and producing hydrogen from solar energy, and to standardize the work in Rhizobials.





<img src="Unamgenomicsassembly.jpg

<img src="Unamgenomicsrsz_operons.jpg

<img src="Unamgenomicsrsz_pberc5.jpg

<img src="UnamgenomicsRsz_psb1c3.jpg

<img src="Unamgenomics6.jpg">

<img src="Unamgenomicsproject1.jpg"