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</html>[[Image:UofC2011_DataPage1.png|thumb|600px|center|<b>Figure 1</b> In order to develop a naphthenic acid biosensor we were required to develop a sensory element which would respond specifically to this class of compounds. Additionally, we developed a novel electrochemical reporter system using the lacZ gene product Beta-galactosidase to detect and quantify the response of our promoter into a measurable system. Since <i>E. coli</i> is not naturally found in tailings ponds, we designed a novel conjugation system to transfer our reporter system into <i>Pseudomonas</i> as well as protocols for use of our system in microalgae.<html> | </html>[[Image:UofC2011_DataPage1.png|thumb|600px|center|<b>Figure 1</b> In order to develop a naphthenic acid biosensor we were required to develop a sensory element which would respond specifically to this class of compounds. Additionally, we developed a novel electrochemical reporter system using the lacZ gene product Beta-galactosidase to detect and quantify the response of our promoter into a measurable system. Since <i>E. coli</i> is not naturally found in tailings ponds, we designed a novel conjugation system to transfer our reporter system into <i>Pseudomonas</i> as well as protocols for use of our system in microalgae.<html> | ||
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Revision as of 21:22, 28 September 2011
TITLE=Building a Naphthenic Acid Biosensor: Summary| BODY=
