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Chloramphenicol stock
From 2011.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | Materials: | + | '''Materials:''' |
Chloramphenicol powder | Chloramphenicol powder | ||
| Line 11: | Line 11: | ||
sterile 15ml centrifuge tube | sterile 15ml centrifuge tube | ||
| - | Procedure: | + | '''Procedure:''' |
| - | 1. Weigh the appropriate amount of antibiotic powder for the amount of ddH2O to be used. (we use 0.165g in 5 ml of H2O to make a 33mg/ml solution.) | + | 1. Weigh the appropriate amount of antibiotic powder for the amount of ddH2O to be used. (''we use 0.165g in 5 ml of H2O to make a 33mg/ml solution.'') |
2. Mix your antibiotic with your ddH2O | 2. Mix your antibiotic with your ddH2O | ||
Latest revision as of 20:40, 28 September 2011
Materials:
Chloramphenicol powder
ddH2O
sterile syringe
syringe filter
sterile 15ml centrifuge tube
Procedure:
1. Weigh the appropriate amount of antibiotic powder for the amount of ddH2O to be used. (we use 0.165g in 5 ml of H2O to make a 33mg/ml solution.)
2. Mix your antibiotic with your ddH2O
3. Absorb your solution with the sterile syringe.
4. Place the syringe filter onto the syringe.
5. Filter your solution into the sterile 15ml centrifuge tube.
