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Team:UC Davis/Notebook wip
From 2011.igem.org
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== Week 1== | == Week 1== | ||
-Rehydrated parts from 2011 Registry Distribution: | -Rehydrated parts from 2011 Registry Distribution: | ||
| - | + | *-R0040=Tet promoter in psb1a2 | |
| - | + | *-C0040=Tet repressor in psb1a2 | |
| - | + | *-C0012=LacI repressor in psb1a2 | |
| - | + | *-C0051=Lambda cI repressor in psb1a2 | |
| - | + | *-R0051=cI-regulated promoter in psb1a2 | |
| - | + | *-I732006=LacZ-alpha in psb1ak3 | |
| - | + | *-R0010=LacI regulated promoter in psb1a2 | |
| - | + | *-E0040=GFP coding region in psb1a2 | |
| - | + | *-J23101=Constitutive promoter in j61002 | |
| - | + | *-C0080=AraC repressor/activator in psb2k3 | |
| - | + | *-I13458=pC+AraC in psb1a3 | |
| - | + | *-I13453=pBAD promoter in psb1a3 | |
| - | + | *-B0015=Double terminator in psb1ak3 | |
-Transformed all hydrated parts | -Transformed all hydrated parts | ||
-Cultured all parts and digested to check on a gel. All parts checked out. Sent out all hydrated parts to get sequenced. | -Cultured all parts and digested to check on a gel. All parts checked out. Sent out all hydrated parts to get sequenced. | ||
Revision as of 19:01, 24 June 2011
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Contents |
Week 1
-Rehydrated parts from 2011 Registry Distribution:
- -R0040=Tet promoter in psb1a2
- -C0040=Tet repressor in psb1a2
- -C0012=LacI repressor in psb1a2
- -C0051=Lambda cI repressor in psb1a2
- -R0051=cI-regulated promoter in psb1a2
- -I732006=LacZ-alpha in psb1ak3
- -R0010=LacI regulated promoter in psb1a2
- -E0040=GFP coding region in psb1a2
- -J23101=Constitutive promoter in j61002
- -C0080=AraC repressor/activator in psb2k3
- -I13458=pC+AraC in psb1a3
- -I13453=pBAD promoter in psb1a3
- -B0015=Double terminator in psb1ak3
-Transformed all hydrated parts -Cultured all parts and digested to check on a gel. All parts checked out. Sent out all hydrated parts to get sequenced. -Ligated mutants into screening plasmid to see if our error-prone PCRs had visible mutants. -Ordered primers for mutagenesis of LacI promoter, cI promoter and tet promoter. Also ordered primers to replace RBS (Bba_B0032) in Bba_E0240 to Bba_B0034.
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
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