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Ampicillin stock
From 2011.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
ddH2O | ddH2O | ||
| + | |||
| + | sterile syringe | ||
| + | |||
| + | syringe filter | ||
| + | |||
| + | sterile 15ml centrifuge tube | ||
| + | |||
| + | Procedure: | ||
| + | |||
| + | 1. Weigh the appropriate amount of antibiotic powder for the amount of ddH2O to be used. (we use 0.25g in 5 ml of H2O to make a 50mg/ml solution. | ||
| + | |||
| + | 2. Mix your antibiotic with your ddH2O | ||
| + | |||
| + | 3. Absorb your solution with the sterile syringe. | ||
| + | |||
| + | 4. Place the syringe filter onto the syringe. | ||
| + | |||
| + | 5. Filter your solution into the sterile 15ml centrifuge tube. | ||
| + | |||
[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''BACK'''] | [https://2011.igem.org/Team:Panama/Protocols/Wetlab '''BACK'''] | ||
Revision as of 20:35, 28 September 2011
Materials:
Ampicillin powder
ddH2O
sterile syringe
syringe filter
sterile 15ml centrifuge tube
Procedure:
1. Weigh the appropriate amount of antibiotic powder for the amount of ddH2O to be used. (we use 0.25g in 5 ml of H2O to make a 50mg/ml solution.
2. Mix your antibiotic with your ddH2O
3. Absorb your solution with the sterile syringe.
4. Place the syringe filter onto the syringe.
5. Filter your solution into the sterile 15ml centrifuge tube.
