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Revision as of 07:04, 28 September 2011 (view source) Ajb5663 (Talk | contribs) (Created page with "==Monday, August 1== J23100+RBS34 Results: no colony growth Colony PCR GFP+tev cleavage site GFP+cI cleavage site LacZα+small linker LacZα+Imp linker LacZ&alpha...") ← Older edit		Revision as of 07:13, 28 September 2011 (view source) Ajb5663 (Talk | contribs) Newer edit →
Line 1:		Line 1:
	==Monday, August 1==		==Monday, August 1==
-	J23100+RBS34	+	Clone LacZα and GusA into K3
-	Results: no colony growth	+
-		+
-	Colony PCR	+
-		+
-	GFP+tev cleavage site	+
-		+
-	GFP+cI cleavage site	+
-		+
-	LacZα+small linker	+
-		+
-	LacZα+Imp linker	+
-		+
-	LacZα+10AA linker	+
-		+
-	XylE+10AA linker	+
-		+
-	Results:GFP+cleavage sites failed, all else worked and were cultured	+
	==Tuesday, August 2==		==Tuesday, August 2==
		+	Colony PCR LacZα and GusA clones
		+	Results: failure
	==Wednesday, August 3==		==Wednesday, August 3==
-		+	Clone GusA and LacZα into K3
	==Thursday, August 4==		==Thursday, August 4==
		+	Colony PCR LacZα and GusA clones
		+	Results: failure
	==Friday, August 5==		==Friday, August 5==
-		+	Place B0015 terminator on tail end of PR-RBS-XylE-small linker-tev cleavage site-GFP
	==Saturday, August 6==		==Saturday, August 6==
-		+	No colonies grew from August 5 assembly
	[[Team:Penn_State/Lab| Back to Wetlab]]		[[Team:Penn_State/Lab| Back to Wetlab]]

---

Revision as of 07:13, 28 September 2011

Contents 1 Monday, August 1 2 Tuesday, August 2 3 Wednesday, August 3 4 Thursday, August 4 5 Friday, August 5 6 Saturday, August 6

Monday, August 1

Clone LacZα and GusA into K3

Tuesday, August 2

Colony PCR LacZα and GusA clones

Results: failure

Wednesday, August 3

Clone GusA and LacZα into K3

Thursday, August 4

Colony PCR LacZα and GusA clones

Results: failure

Friday, August 5

Place B0015 terminator on tail end of PR-RBS-XylE-small linker-tev cleavage site-GFP

Saturday, August 6

No colonies grew from August 5 assembly Back to Wetlab