Team:WashU/Modeling

From 2011.igem.org

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(Modeling)
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[[File:Pathway1.jpg|thumb|left|upright=3]]
[[File:Pathway1.jpg|thumb|left|upright=3]]
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The pathway for producing beta-carotene. Yeast naturally produce GGPP but no other intermediates in beta-carotene pathway, crtYB and crtI are needed to to complete the pathway for beta-carotene production. Notice crtYB codes for two proteins needed in the pathway. CrtE further improves beta-carotene production as it aides in the production of the starting product, GGPP.
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The pathway for producing beta-carotene as shown in "High-Level Production of Beta-Carotene in Saccharomyces cerevisiae by Successive Transformation with Carotenogenic Genes from Xanthophyllomyces dendrorhous" by Verwaal, Rene et. Al. Yeast naturally produce GGPP but no other intermediates in beta-carotene pathway, crtYB and crtI are needed to to complete the pathway for beta-carotene production. Notice crtYB codes for two proteins needed in the pathway. CrtE further improves beta-carotene production as it aides in the production of the starting product, GGPP.
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Revision as of 01:47, 28 September 2011





Modeling

Pathway1.jpg

The pathway for producing beta-carotene as shown in "High-Level Production of Beta-Carotene in Saccharomyces cerevisiae by Successive Transformation with Carotenogenic Genes from Xanthophyllomyces dendrorhous" by Verwaal, Rene et. Al. Yeast naturally produce GGPP but no other intermediates in beta-carotene pathway, crtYB and crtI are needed to to complete the pathway for beta-carotene production. Notice crtYB codes for two proteins needed in the pathway. CrtE further improves beta-carotene production as it aides in the production of the starting product, GGPP.








Pathway2.jpg

The pathway for producing beta-ionone from beta-carotene. This process only involves the expression of one gene, CCD, which codes for enzymatic oxidative cleavage 1 and 2, which cleaves the ends of beta-carotene to produce two beta-ionone molecules.









MasterPlan1.jpg