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Team:UC Davis/LacI
From 2011.igem.org
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<h2>DNA Sequence</h2> | <h2>DNA Sequence</h2> | ||
<center> | <center> | ||
| + | <img src="https://static.igem.org/mediawiki/2011/d/d9/UCD_Mut_graph_lacI.png"> | ||
<img src="https://static.igem.org/mediawiki/2011/9/9f/UCD_R10mut_seq_REDMUTS_09242011.png"> | <img src="https://static.igem.org/mediawiki/2011/9/9f/UCD_R10mut_seq_REDMUTS_09242011.png"> | ||
| - | </center> | + | </center><br><br> |
| + | The sequences above show our 7 LacI mutants. There are between 1 and 7 mutations in each sequence as indicated by the red bases. All 7 sequences have mutations between bases 100 and 200 which contains the known locations of the CAP binding site(bases 88-127) and LacI binding site (bases 166-200). | ||
</div> | </div> | ||
Revision as of 03:40, 28 September 2011
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LacI
The lac repressor is responsible for regulating the metabolism of lactose. In the absence of lactose, LacI forms a tetramer with identical subunits which appears as two dimers. Each dimer binds in the major groove of the DNA binding region which subsequently blocks the RNA polymerase from binding. In nature, allolactose will bind the repressor leading to transcription of the lac operon. Using IPTG as an inducer has the same effect as allolactose.
To the right is a small render of the LacI tetramer bound to its operator.
Construct
DNA Sequence
The sequences above show our 7 LacI mutants. There are between 1 and 7 mutations in each sequence as indicated by the red bases. All 7 sequences have mutations between bases 100 and 200 which contains the known locations of the CAP binding site(bases 88-127) and LacI binding site (bases 166-200).
