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Team:UC Davis/LacI
From 2011.igem.org
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<h1>LacI</h1> | <h1>LacI</h1> | ||
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| - | The lac repressor is responsible for regulating the metabolism of lactose. In the absence of lactose, LacI forms a tetramer with identical subunits which appears as two dimers. Each dimer binds in the major groove of the DNA binding region which subsequently blocks the RNA polymerase from binding. <iframe src="http://player.vimeo.com/video/27753866?title=0&byline=0&portrait=0" width="400" height="300" frameborder="0" style="float:right"></iframe> In nature, allolactose will bind the repressor leading to transcription of the lac operon. Using IPTG as an inducer has the same effect as allolactose. | + | <p>The lac repressor is responsible for regulating the metabolism of lactose. In the absence of lactose, LacI forms a tetramer with identical subunits which appears as two dimers. Each dimer binds in the major groove of the DNA binding region which subsequently blocks the RNA polymerase from binding. <iframe src="http://player.vimeo.com/video/27753866?title=0&byline=0&portrait=0" width="400" height="300" frameborder="0" style="float:right"></iframe> In nature, allolactose will bind the repressor leading to transcription of the lac operon. Using IPTG as an inducer has the same effect as allolactose. </p> |
| - | + | <p>To the right is a small render of the LacI tetramer bound to its operator.</p> | |
</div> | </div> | ||
Revision as of 01:20, 28 September 2011
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LacI
The lac repressor is responsible for regulating the metabolism of lactose. In the absence of lactose, LacI forms a tetramer with identical subunits which appears as two dimers. Each dimer binds in the major groove of the DNA binding region which subsequently blocks the RNA polymerase from binding. In nature, allolactose will bind the repressor leading to transcription of the lac operon. Using IPTG as an inducer has the same effect as allolactose.
To the right is a small render of the LacI tetramer bound to its operator.
Construct
DNA Sequence
